醛固酮对心室成纤维细胞分泌内皮素的影响  

Effect of aldosterone on the secretion of endothelin by ventricular fibroblasts

作　　者：龚素珍[1] 刘培庆[1] 鲁伟[1] 王庭槐[1] 符史干[1] 谈智[1] 潘敬运[1] 

机构地区：[1]中山医科大学生理学教研室,广州510089

出　　处：《生理学报》2001年第1期23-26,共4页Acta Physiologica Sinica

摘　　要：用细胞培养、内皮素放射免疫测定和RT PCR的方法 ,探讨醛固酮对心室成纤维细胞分泌内皮素的影响。结果显示 ,醛固酮 (1× 10 -7mol/L)能促进心室成纤维细胞ppET 1mRNA的表达 ,在作用后 2h ,表达开始增加 ,4h达高峰 ,以后逐渐下降 ;同时醛固酮能增加成纤维细胞内和条件培养液中内皮素的含量。其受体阻断剂螺旋内酯 (1× 10 -6mol/L)能拮抗醛固酮的作用 ,提示醛固酮能促使心室成纤维细胞分泌内皮素 。Using cell culture, radioimmunoassay for endothelin and RT PCR, the effect of aldosterone on the endothelin secretion of ventricular fibroblasts was studied. The results showed that aldosterone (1×10 -7 mol/L) promoted the expression of ppET 1 mRNA, which began to increase in 2 hours and attained the highest level in 4 hours, thereafter decreased; aldosterone increased the endothelin level in ventricular fibroblasts and fibroblast conditioned growth medium (FCGM) as well, which was blocked by spironolactone (1×10 -6 mol/L), an aldosterone receptor antagonist. The results suggest that aldosterone can increase endothelin secretion by ventricular fibroblasts, which can be inhibited by its receptor antagonist spironolactone.

关 键 词：醛固酮 螺旋内酯 内皮素放射免疫测定 ppET-1mRNA 心室成纤细胞 内皮素 

分 类 号：R363.1[医药卫生—病理学]