内毒素引起的乳鼠心肌细胞血红素加氧酶-1基因的表达  

Expression of heme oxygenase-1 in neonatal rat cardiocytes induced by lipopolysaccharide

作　　者：李玉明[1] 刘俊昌[2] 张敏[2] 郑新程[2] 吴立玲[2] 时安云[2] 伍贻经[2] 

机构地区：[1]承德医学院低温生理研究室,承德067000 [2]北京医科大学病理生理学教研室,北京100083

出　　处：《生理学报》2001年第1期37-40,共4页Acta Physiologica Sinica

基　　金：ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina (No 396 70 30 8)

摘　　要：为了探讨在内毒素作用下的乳鼠心肌细胞 (neonatalratcardiomyocytes,NRCMs)血红素加氧酶 1(hemeoxyge nase 1,HO 1)基因的表达及其在细胞损伤中的作用 ,分别用 10、30及 5 0 μg/ml的脂多糖 (lipopolysaccharide ,LPS) ,10μg/mlLPS + 10 μmol/ml锌原卟啉Ⅸ (Zn protoporphyrin Ⅸ ,ZnPPⅨ )和单纯 10 μmol/mlZnPPⅨ与培养的NRCMs共同孵育 6h ,以及 10 μg/mlLPS与NRCMs共同孵育 9h和 18h。分别观察细胞HO 1mRNA表达、MDA含量、LDH释放量与台盼蓝摄取率的变化。结果显示 ,同样与细胞孵育 6h ,LPS 10 μg/ml时HO 1mRNA表达比对照组增加 81 2 % ,30μg/ml时表达量增加 12 6 3% ,5 0 μg/ml时表达量增加 92 8% ;LPS为 10 μg/ml时 ,孵育 9h后HO 1mRNA的表达量比对照组增加 93 6 % ,孵育 18h后表达量增加 10 5 8%。LPS 30、5 0 μg/ml,10 μg/mlLPS + 10 μmol/mlZnPPⅨ与细胞孵育6h及LPS 10 μg/ml孵育 18h后 ,细胞MDA含量、LDH释放量与台盼蓝摄取率明显增加 (P <0 0 1) ;单纯 10 μg/mlLPS与单纯 10 μmol/mlZnPPⅨ孵育 6h后 ,上述指标均无明显升高。结果表明 ,LPS可诱导NRCMsHO 1mRNA的表达 ,且在较低LPS剂量范围内具有时间依赖性和浓度依赖性 ;NRCMsHO 1mRNA的表达可减低LPS引起的细胞损伤 。To study the alterations of heme oxygenase 1 mRNA in neonatal rat cardiomyocytes (NRCMs) induced by lipopolysaccharide (LPS) and the role of heme oxygenase 1 (HO 1) in the LPS induced disorders of myocardium function, 10 (L, 6 h), 30 (M, 6 h), 50 μg/ml (H, 6 h) LPS and 10 μg/ml LPS +10 μmol/ml Zn protoporphyrin Ⅸ (ZnPPⅨ; L+I, 6 h) and 10 μmol/ml ZnPPⅨ alone (I, 6 h) were added to the medium for a 6 hour culture of NRCMs, and 10 μg/ml LPS for 9 h (L, 9 h) and 18 h (L, 18 h) cultures. LDH release and MDA contents of the cells were measured. When NRCMs were collected, Trypan blue stain method was used to examine the mortality (the rate of Trypan blue uptake) of NRCMs. HO 1 mRNA expression was examined by Northern blot. The results showed that HO 1 mRNA expression of NRCMs increased gradually along with the increase of LPS concentration below the level of 30 μg/ml. When the final concentrations of LPS were 10 and 30 μg/ml, the HO 1 mRNA expression of NRCMs increased by 81 2% and 126 3% respectively compared with control. When the final concentration of LPS was 50 μg/ml, the HO 1 mRNA expression decreased to the level of 10 μg/ml group. When the final concentration was 10 μg/ml, the HO 1 mRNA expression increasedｇｒａｄｕａｌｌｙａｌｏｎｇｗｉｔｈｔｈｅｃｕｌｔｕｒｅｔｉｍｅ .Ａｆｔｅｒａ 9 ｏｒ 18 ｈｏｕｒｃｕｌｔｕｒｅ ,ｔｈｅＨＯ 1ｍＲＮＡｅｘｐｒｅｓｓｉｏｎｏｆＮＲＣＭｓｉｎ ｃｒｅａｓｅｄｂｙ 93 6%ａｎｄ 10 5 8%ｒｅｓｐｅｃｔｉｖｅｌｙｃｏｍｐａｒｅｄｗｉｔｈｃｏｎｔｒｏｌ.ＯｎｌｙｗｈｅｎＮＲＣＭｓｈａｄｂｅｅｎｃｕｌｔｕｒｅｄｗｉｔｈ30 ,50 μｇ/ｍｌＬＰＳａｎｄ 10 μｇ/ｍｌＬＰＳ +10 μｍｏｌ/ｍｌＺｎＰＰⅨｆｏｒ 6ｈａｎｄ 10 μｇ/ｍｌＬＰＳｆｏｒ 18ｈ ,ｔｈｅｒａｔｅｏｆＴｒｙｐａｎｂｌｕｅｓｔａｉｎｕｐｔａｋｅ ,ＭＤＡｃｏｎｔｅｎｔｓａｎｄ

关 键 词：内毒素 血红素加氧酶 基因表达 心肌细胞 

分 类 号：R364[医药卫生—病理学]