蛙皮素-蜂毒素杂合肽基因在大肠杆菌中的克隆与诱导表达  

Cloning and Expression of a Synthetic Gene Encoding Magainin-Melittin Hybrid Peptide in Escherichia coli and Studies on its Antibacterial Activity

作　　者：黄音[1] 刘飞鹏[2] 周天鸿[2] 朱嘉明[2] 

机构地区：[1]上海复旦大学遗传所 [2]暨南大学生物工程系,广州510632

出　　处：《生物工程学报》2001年第2期207-210,共4页Chinese Journal of Biotechnology

基　　金：广东省自然科学基金!资助 (970 6 36 )

摘　　要：A hybrid peptide gene was designed and synthesized. Its encoding peptide is constructed from residues 3～14 of magainin and residues 1～13 of melittin. The MA E gene was cloned into plasmids pUC18 and pBV220. By DNA sequencing, the whole sequences of this gene is confirmed to be correct. The recombinant plasmid pBMA\|E was expressed in \%E.coli\% DH5α. A gene product band can be seen with Tricine\|SDS\|PAGE. The MA E hybrid peptide was purified by immobilized metal affinity chromatography. Bioactivity assay was carried out in liquid turbidity method. The bactericide value to \%E.coli\% K 12 D 31 is 0.182.A hybrid peptide gene was designed and synthesized. Its encoding peptide is constructed from residues 3～14 of magainin and residues 1～13 of melittin. The MA E gene was cloned into plasmids pUC18 and pBV220. By DNA sequencing, the whole sequences of this gene is confirmed to be correct. The recombinant plasmid pBMA\|E was expressed in \%E.coli\% DH5α. A gene product band can be seen with Tricine\|SDS\|PAGE. The MA E hybrid peptide was purified by immobilized metal affinity chromatography. Bioactivity assay was carried out in liquid turbidity method. The bactericide value to \%E.coli\% K 12 D 31 is 0.182.

关 键 词：抗菌肽 MA-E基因 大肠杆菌 抗菌活性 表达 克隆 蛙皮素-蜂毒素杂合肽基因 

分 类 号：Q786[生物学—分子生物学]