普通小麦穗发芽抗性相关分子标记在RIL群体中的验证与评价  

Evaluation and Validation of Molecular Marker Associated with Pre-harvest Sprouting Tolerance in a RIL Population

作　　者：马丽[1] 李治[1] 任天恒[2] 唐宗祥[2] 晏本菊[1,2] 任正隆[2] 

机构地区：[1]四川农业大学生命科学与理学院成都教学部,四川成都611130 [2]四川农业大学植物遗传育种省级重点实验室,四川成都611130

出　　处：《麦类作物学报》2014年第4期435-442,共8页Journal of Triticeae Crops

基　　金：国家自然科学基金重点项目(30730065);四川省教育厅育种专项(2011-2015)

摘　　要：为了鉴定我国西南冬麦区普通小麦品种的穗发芽抗性,筛选能鉴定穗发芽抗性的相关分子标记,利用小麦品种川农17和新品系R146构建的重组自交系（F7：8）共135个家系作为研究材料,通过测定种子的发芽指数和降落值来共同鉴定小麦的穗发芽抗性。选择7个已发表的与穗发芽抗性相关的分子标记（Xgwm46、Xgwm269、Xgwm282、Xgwm328、Xgwm397、Xwmc468和Xgwm518）对这些材料进行PCR扩增,分析扩增片段与发芽指数和降落值的相关性。结果表明,在135份重组自交系材料中,发芽指数小于0.4的材料有21份,介于0.4～0.8的材料有86份,高于0.8的材料有28份;降落值小于250的材料有20份,大于400的有16份。标记扩增片段与发芽指数和降落值相关分析表明,7个标记中有3个标记（Xgwm397、Xgwm282和Xwmc468）与穗发芽抗性相关,标记Xgwm397和Xwmc468可作为穗发芽抗性选择的分子标记在育种中加以利用;另外三个标记Xgwm269、Xgwm328、Xgwm518与穗发芽抗性不相关;标记Xgwm46只与发芽指数相关,与降落值相关不显著,能否用作穗发芽筛选标记需进一步验证。To identify the breeding materials with pre-harvest sprouting（PHS）tolerance and to screen the efficient markers associated with PHS tolerance,in the study,the germination index and falling number were measured in a set of 135recombinant inbred lines（F7：8）which derived from a cross between Chuannong 17（CN17）and R146,and were used to analyze their correlations with the PCR amplification patterns of seven makers（Xgwm46,Xgwm269,Xgwm282,Xgwm328,Xgwm397, Xwmc468 and Xgwm518）associated with PHS tolerance.The results showed that among the 135recombinant inbred lines tested,the germination index of 21lines were lower than 0.4,that of 86lines were between 0.4and 0.8,and 28lines were over 0.8;the falling number of 20lines were lower than 250,that of 16lines were over 400.The relationship between the variations of PCR products of the seven makers with the germination index and falling number of the tested lines confirmed that threemarkers of Xgwm397,Xgwm282,Xwm468 were associated with PHS response,but that of Xgwm269,Xgwm328,Xgwm518 were not,and whether the maker Xgwm46 was associated with PHS response needed further study.

关 键 词：小麦 穗发芽抗性 降落值 发芽指数 分子标记 

分 类 号：S512.1[农业科学—作物学] S332