普通小麦-中间偃麦草易位系08-738的鉴定  

Identification of a Translocation Line 08-738 between Common Wheat-Thinopyrum intermedium

作　　者：李文静[1] 葛群[1] 王仙[1] 唐雪琴[1] 徐杰[1] 唐宗祥[1] 任正隆[1] 傅体华[1] 

机构地区：[1]四川农业大学农学院,四川成都611130

出　　处：《麦类作物学报》2014年第4期443-448,共6页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(30370770);四川省教育厅重点项目

摘　　要：中间偃麦草(Thinopyrum intermedium,2n=42)具有大穗多花和抗多种病害等特性,是小麦育种的重要基因资源之一。为确定普通小麦川麦107与中间偃麦草杂交获得的遗传稳定品系08-738的染色体组成,采用形态学、细胞遗传学和SSR分子标记对其进行了鉴定。形态学分析表明,08-738具有植株较矮和小穗数较多的特点。细胞学观察表明,其染色体数目及构型为2n=42=21II。基因组原位杂交(GISH)和重复序列原位杂交(FISH)结果表明,08-738含有20对小麦染色体和1对小麦-中间偃麦草小片段易位染色体,易位位于小麦3DS的近末端,且该外源片段可能来源于中间偃麦草的Js染色体组。SSR标记分析显示,位于3DS 6-0.55-1.00之间的SSR标记xcfd141能在08-738和中间偃麦草之间扩增出一条特异条带,xcfd141可作为该中间易位片段鉴定和选择的标记。Thinopyrum intermedium（2n = 42）is an important genetic resource for wheat improvement due to its large and multiflorous spikes and resistances to various wheat diseases.In order to accurately identify the chromosome constitution of the genetic stable wheat line 08-738derived from the progenies of Chuanmai 107and Thinopyrum intermedium,analyses on the morphology,cytology and SSR marker on wheat line 08-738were carried out.The result of morphology showed that line 08-738 had shorter plant height and more spikelets per spike than its recurrent wheat parent.Cytogenetic analysis indicated that this line was stable with chromosome number and configuration of 2n=42= 21II.The sequential GISH and FISH analysis indicated that the chromosome constitution of line 08-738consisted of 20pairs of wheat chromosomes and one pair of small segmental translocation chromosomes of wheat and Th.intermedium,and the translocation segment was closed to telomere of wheat 3DS and might be derived from the Js genome.The SSR marker analysis showed that SSR primer xcfd141located on 3DS 6-0.55-1.00could amplify one specific band between line 08-738and Th.intermedium L.Thus,primer xcfd141could be used as the marker to select and identify this small translocation segment in wheat background.

关 键 词：小麦 中间偃麦草 易位 GISH FISH SSR 

分 类 号：S512.1[农业科学—作物学] S334