强啡肽A_(1-13)对谷氨酸诱导的大鼠C6胶质瘤细胞肿胀的影响  

EFFECT OF DYNORPHIN A_( 1-13) ON C6 GLIOMA CELLS SWELLING INDUCED BY GLUTAMATE

作　　者：孙学军[1] 王雪琦[1] 王成海[1] 路长林[1] 

机构地区：[1]第二军医大学神经科学研究所,神经生物学教研室上海200433

出　　处：《中国应用生理学杂志》2001年第1期76-78,共3页Chinese Journal of Applied Physiology

摘　　要：目的和方法 :探讨脑水肿发病的细胞机制 ,采用 [3H]OMG摄取的方法测定细胞含水量 ,观察DynA对谷氨酸诱导的大鼠C6胶质瘤细胞肿胀的影响。结果 :①给予 0 .5 ,1.0 ,10 .0mmol/L的谷氨酸作用 1h均可引起细胞的含水量增加 ;②DynA可显著降低谷氨酸诱导肿胀的大鼠C6胶质瘤细胞含水量 ;③κ阿片受体拮抗剂nor BNI可阻断DynA1-13 降低谷氨酸诱导肿胀的大鼠C6胶质瘤细胞含水量的作用。结论 :谷氨酸可诱导大鼠C6胶质瘤细胞肿胀 ;DynA1-13Aim and Method:To explore the cell mechnism of brain edema and the effect of dynorphin A 1-13 on swelling of C6 glioma cells.Water content of cell are studied by using 3 O methyl D glucose.Results:①Glutamate(0.5,1.0,10.0 mmol/L)increased the water content of C6 glioma at an hour.② Dynorhin A 1-13 could significantly decrease the increasing in water content of C6 glioma cells induced by glutamate.③nor-BNI,a antagonist,could inhibit the effect of dynopbin A 1-13 on water content of swelling C6 glioma cells.Conclusion:Glutamate could induce the swelling of C6 glioma cells.Dynorphin A 1-13 could reduce C6 glioma cells swelling induced by glutamate via kappa opioid receptor.

关 键 词：强啡肽A1-13 谷氨酸 C6胶质瘤细胞 细胞肿胀 脑水肿 

分 类 号：R742.7[医药卫生—神经病学与精神病学] R739.4[医药卫生—临床医学]