高压氧对脑缺血再灌注海马CA_1区神经元凋亡作用的研究  

STUDY ON THE EFFECT OF HYPERBARIC OXYGEN ON APOPTOSIS IN THE CA_1 REGION OF HIPPOCAMPUS FOLLOWING FOREBRAIN ISCHEMIA REPERFUSION

作　　者：周建光[1] 刘景昌[1] 方以群[1] 

机构地区：[1]海军医学研究所,上海200433

出　　处：《中国应用生理学杂志》2001年第1期82-84,共3页Chinese Journal of Applied Physiology

基　　金：中国人民解放军总后勤部卫生部科研基金资助课题!(96L0 0 3)

摘　　要：目的和方法 :应用TUNEL检测技术 ,对沙土鼠前脑缺血 2 0min后再灌注 3d模型 ,用HBO治疗连续 3d。观察HBO作用下海马CA1区神经元凋亡变化 ,研讨HBO对脑缺血再灌注损伤的疗效及其机理 ,为临床应用HBO治疗疾病提供理论依据。结果 :沙土鼠脑缺血再灌注 3d后海马CA1区大量神经元凋亡 ,HBO治疗组凋亡细胞数明显减少 (P <0 .0 1) ,并以 0 .2 5MPaHBO治疗组为佳。结论 :HBO治疗对海马神经元损伤有保护作用 ,减少神经元凋亡 。Aim and Methods: To study the mechanism of hyperbaric oxygen (HBO) on the treatment of cerebral ischemia, and to provide theoretical basis for hyperbaric oxygen treatment (HBOT), we examined the effect of 0.15 MPa and 0.25 MPa HBO treatment (60 min/d, for 3 successive days ) on ischemia reperfusion-induced apoptosis in gerbil hippocampus CA 1 using TUNEL staining method.Results: The number of neuronal apoptosis in hippocampus CA 1 was significantly decreased in HBOT groups ( P <0.01). Neuronal apoptosis in hippocampus CA 1 in 0.25 MPa groups were less than that in 0.15 MPa groups ( P <0.01). Conclusion: HBOT is effective in reducing neuronal apoptosis and in protecting neurons from injury of ischemia reperfusion, and 0.25 MPa is an appropriate pressure for HBOT.

关 键 词：高压氧 脑缺血再灌注 凋亡 海马CA1区 神经元 

分 类 号：R743.31[医药卫生—神经病学与精神病学]