应激状态下大鼠咬肌活动性与颞下颌关节病变的相关性分析  

Correlation analysis between masseter muscle activity and temporomandibular joint disease in stressed rats

作　　者：肖朋[1] 王兵[1] 黄旭[1] 刘海霞[1] 王虹[2] 

机构地区：[1]新疆医科大学第二附属医院口腔科,乌鲁木齐830028 [2]新疆医科大学附属中医医院口腔科,乌鲁木齐830026

出　　处：《国际口腔医学杂志》2014年第4期406-408,共3页International Journal of Stomatology

基　　金：国家自然科学基金(81360169)

摘　　要：目的探讨心理应激状态下大鼠咬肌异常活动度与颞下颌关节病变程度之间的相关性。方法选取36只成年Wistar大鼠,随机分为3组,即实验3周组、实验6周组和空白对照组,每组12只。对实验3周组和实验6周组分别施加预定刺激3周和6周,使大鼠处于应激状态。处死3组动物,分别取大鼠颞下颌关节和咬肌组织,制作关节病理切片,观察关节组织结构病理变化;定量咬肌组织肌肉匀浆检测乳酸脱氢酶(LDH)和肌酸激酶(CK)含量,并分析每份标本中咬肌LDH、CK含量与颞下颌关节病变程度之间的相关性。结果应激状态下大鼠咬肌肌肉中LDH、CK含量与颞下颌关节病变程度呈正相关;应激状态下大鼠咬肌肌肉中LDH、CK含量影响颞下颌关节病变程度。结论应激状态下咬肌异常活动度有可能作为大鼠颞下颌关节病理变化程度的参考指标,咬肌异常活动可能是致颞下颌关节病变的途径之一。Objective This study aims to investigate the correlation between the rat masseter abnormal activity and the degree of pathological changes of temporomandibular joint disease under mental stress. Methods A total of 36 adult Wistar rats were randomly divided into three groups： three-week experimental, six-week experimental, and control groups（n=12 for each group）. A predetermined stimulus was applied on the experimental groups to place the rats in a stress state. The three rat groups were then sacrifi ced. The rat temporomandibular joint and masseter muscle tissue were obtained and submitted to the joint pathological section for observation of the pathological changes of the joint organizational structure. The levels of lactate dehydrogenase（LDH） and creatine kinase（CK） were quantitatively determined in the masseter muscle tissue homogenates. Spearman correlation and multiple regression analyses were used for statistical analysis. Analysis of the CK and LDH contents in each specimen masseter muscle was carried out to determine their correlation to the severity of the temporomandibular joint. Results A positive correlation was observed between the LDH and CK contents in the masseter muscle of rats to the severity of the temporomandibular joint. The levels of LDH and CK in the masseters of stressed rats showed a reciprocal relationship to the extent of temporomandibular joint disease. Conclusion The masseter muscle abnormal activity under stress may be used as a reference indicator of the degree of pathological changes of the rat temporomandibular joint. The masseter muscle abnormal activity is possibly caused by a temporomandibular joint disease.

关 键 词：应激 颞下颌关节 咬肌活动性 

分 类 号：R782.6[医药卫生—口腔医学]