高浓度胰岛素对阿司匹林抑制的血小板中TXB2合成影响的研究  

作　　者：高玉龙[1] 龙炫辉 田平阁[2] 高德禄[1] 

机构地区：[1]南方医科大学研究生学院,510515 [2]中国人民解放军305医院中心实验室,100017

出　　处：《浙江临床医学》2014年第6期853-855,共3页Zhejiang Clinical Medical Journal

基　　金：全军十一五计划科技攻关基金资助项目（09BAk61801）

摘　　要：目的：探讨高浓度胰岛素是否影响阿司匹林抑制的血小板中TXB2合成。方法将制备的两组等体积的富含血小板血浆（PRP）分别加入浓度为30μU/ml和300μU/ml的胰岛素，37℃的环境下孵育3min，再次将上述两组的PRP分成等体积，分别用浓度为0、75μmol/L、150μmol/L和300μmol/L的阿司匹林处理，于37℃的环境下继续孵育30min，加入ADP 5min后，用吲哚美辛和柠檬酸葡萄糖封闭血小板反应；用血小板聚集仪检测各组的血小板最大聚集率，酶联免疫吸附法检测血小板中TXB2的含量。结果胰岛素300μU/mL组与30μU/mL组比较，前者可明显升高经阿司匹林作用后的血小板最大聚集率（P〈0.05），且明显增加血小板中TXB2的合成（P〈0.05）。结论高浓度胰岛素可减弱阿司匹林抑制的血小板TXB2合成作用。Objective In this study,we examined if acute exposure to increased plasma insulin impaires the inhibitory effects of aspirin on thromboxane B2 synthesis. Methods The equivalent volume of Platelet-rich plasma（PRP） prepared by hand was preincubated with insulin（30 ,300μU/ml） in 37℃water for 3 minutes,which was then stimulated with different concentrations of aspirin（0,75,150,300μmol/L）for 30 minutes in water of 37℃. In PRP,TXB2 levels were measured at the end of the aggregation tests（i.e.,5min after the addition of agonists-ADP）after blocking platelet responses with indomethacin and citrate-dextrose. The platelet maximal aggregation each sample were analyzed by platelet aggregation system. TXB2 was measured by using the EIA kit. Results Insulin 300μU/ml significantly attenuated the inhibitory effects of aspirin on ADP-induced platelet aggregation and thromboxane B2 compared with the group of insulin 30μU/mL. Conclusion In vitro exposure to high insulin（i.e.,300μU/mL）of platelets reduces the thromboxane B2 synthesis action of aspirin.

关 键 词：胰岛素 阿司匹林 血小板 血小板聚集率 血栓烷B2 THROMBOXANE B2 

分 类 号：R457.1[医药卫生—治疗学]