三品系小型猪35个微卫星基因座的遗传学研究  

Genetic Analysis of 35 Microsatellite Loci in Three Miniature Pig Breeds

作　　者：商海涛[1] 牛荣[1] 魏泓[1] 黄中波[1] 甘世祥[2] 王爱德[3] 曾养志[4] 

机构地区：[1]第三军医大学实验动物中心,重庆400038 [2]贵阳中医学院实验动物研究所,贵阳550002 [3]广西大学动物科学技术学院,南宁530005 [4]云南农业大学遗传资源研究室,昆明650201

出　　处：《遗传》2001年第1期17-20,共4页Hereditas（Beijing)

基　　金：国家重点基础研究发展规划项目! (G2 0 0 0 0 16 10 6 );国家"九五"重点科技攻关项目! (96A -2 3 -0 6 -0 1);国家自然科学基金重

摘　　要：利用 35个微卫星基因座对中国三个品系的小型猪 (贵州小型香猪、广西巴马小型猪、版纳小耳猪近交系 )进行了遗传检测。计算出三个小型猪品系个体样本在 35个微卫星基因座的纯合率 ,并对其进行t检验。计算出各品系的平均杂合度 ,多态信息含量 (PIC)及品系间的遗传距离 ,并进行了系统聚类。结果表明三个品系的小型猪其基因纯合率均较高 ,其中版纳小耳猪近交系的基因纯合率最高 ;PIC和平均杂合度均较低 ;贵州小型香猪和广西巴马小型猪亲缘关系较近 ,并均与版纳小耳猪近交系的亲缘关系略远。The polymorphism of 35 microsatellites in the three miniature pig breeds in China(Guizhou miniature pig, Guangxi Bama miniature pig, Banna miniature pig inbred) was analysed. Rates of homozygote for 35 microsatellite loci in three miniature pig breeds were calculated,and t-test for them were performed. Mean heterozygosity and polymorphism information content(PIC) were calculated for all breeds, and genetic distances between these breeds were estimated. The dendrograms were obtained based on genetic distances. The results suggest that rates of homozygote in the three breeds are all high, and that is the highest in Banna miniature pig inbred. The results also suggest that polymorphism information content and mean heterozygosity in all the three breeds are low, and the genetic relationship between Guizhou miniature pig and Guangxi Bama miniature pig is closer than their relationship with Banna miniature pig inbred.

关 键 词：小型猪 微卫星 亲缘关系 遗传学 

分 类 号：S828[农业科学—畜牧学]