KH34基因荧光表达质粒的构建  

Construction of fluorescence expression plasmid of KH34 gene

作　　者：李德玲[1] 刘欣[1] 顾巍[1] 

机构地区：[1]汕头大学医学院病理生理学实验室,广东汕头515041

出　　处：《中国处方药》2014年第6期41-42,共2页Journal of China Prescription Drug

摘　　要：目的构建KH34基因的荧光表达质粒。方法应用分子生物学手段将KH34基因克隆入phage-UBC-flag-cherry载体,构建KH34的荧光表达质粒,并以该质粒转染293T细胞,荧光显微镜下观察该质粒在细胞内的定位。结果成功构建了KH34的荧光表达质粒,并成功转染293T细胞。结论本实验成功构建了KH34的荧光表达质粒,转染后的293T细胞也具有明显的红色荧光,为后续分析KH34对细胞的功能影响奠定了基础。Objective Construction of fluorescence expression plasmid of KH34 gene. Methods Application of molecular biology methods, The KH34 gene was cloned into phage-UBC-flag-cherry vector to construct the fluorescence expression plasmid of KH34, and then the plasmid was transfected into 293T cells, observing the location of the plasmid in 293T cells under fluorescence microscope. Results The fluorescence expression plasmid of KH34 gene was constructed, and it was transfected into 293T cells. Conclusion We successfully constructed the fluorescence expression plasmid of KH34 gene, and there was visibly fluorescence in the transfected 293T cells, this laid a foundation for the subsequent analysis of KH34 on cell function.

关 键 词：KH34 荧光 质粒构建 荧光显微镜 

分 类 号：R346[医药卫生—基础医学]