排风藤提取物中东莨菪苷对神经细胞的抗氧化活性研究  

Antioxidant Activity of Scopolin from Solanum cathayanum to SH-SY5Y cells

作　　者：孔玉珊 黄少兰[1] 谢明霞[1] 薛艳红[1] 邹坤[1] 刘士平[1] 

机构地区：[1]天然产物研究与利用湖北省重点实验室三峡大学生物与制药学院,宜昌443002

出　　处：《天然产物研究与开发》2014年第6期943-946,共4页Natural Product Research and Development

基　　金：国家自然科学基金项目(31270389;31070313);湖北省教育厅自然科学研究项目(D20121301)

摘　　要：前期研究显示排风藤提取物具有较好的抗氧化活性,为进一步明确其活性成分与功效,本文经过提取分离、结构鉴定后,探讨了其单体化合物东莨菪苷对H2O2所致人神经母细胞瘤株SH-SY5Y细胞系氧化应激损伤后的抗氧化保护作用。结果表明在远离其毒性范围内,东莨菪苷呈现出与阳性对照维生素E相近的抗氧化活性,LDH漏出率明显降低,说明东莨菪苷具有降低膜损伤的作用;此外,0.1μM的东莨菪苷将有助于细胞提高超氧化物歧化酶、谷胱甘肽等抗氧化物质的含量,表明其可以作为一种潜在的抗神经退行性疾病药物进行开发和利用。Solanum cathayanum extracts showed obviously antioxidant activity in previous study. Here,in order to further investigate the active components of S. cathayanum, the monomeric compound scopolin was isolated, purified and structurally identified. The antioxidant effects of scopelin to SH-SYSY cells under H202 oxidative stress were assessed. The results revealed that scopelin had evident antioxidant activity in the nontoxic concentration, similar to that of the positive control vitamin E. Besides, LDH leakage rate decreased obviously, suggested that scopolin had the ability of protecting membrane from injury. 0. 1 μM of seopolin helped the cells to improve antioxidant content such as superoxide dismutase （SOD） and glutathione （GSH）. All the results indicated that scopolin can be developed as a potential anti-nerve degen- erative disease drug.

关 键 词：排风藤 东莨菪苷 H2O2 SH-SY5Y细胞 抗氧化活性 

分 类 号：R961[医药卫生—药理学] Q946.8[医药卫生—药学]