黄芩苷对实验性结肠炎小鼠TLRs/MyD88通路的作用研究  

Baicalin Improves DSS-Induced Colitis in Mice Via the TLRs /MyD88 Signaling Pathway

作　　者：邹颖[1,2] 迟宏罡 欧阳霖芮 石敏[3] 蔡碧茉 伍俏璐 戴世学[4] 冯锦山 赵兵 郑学宝 

机构地区：[1]东医学院第二临床医学院中医学教研室 [2]广东医学院中美肿瘤研究所 [3]广东医学院药学院,东莞523808 [4]南方医科大学南方医院急诊科,广州510515

出　　处：《天然产物研究与开发》2014年第6期952-956,共5页Natural Product Research and Development

基　　金：国家自然科学基金项目(81173240);广东省自然科学基金项目(10152402301000003)

摘　　要：本文研究黄芩苷对葡聚糖硫酸钠(DSS)诱导的结肠炎小鼠模型的疗效,并从TLRs/MyD88通路探讨其作用机制。C57BL/6小鼠24只,分为空白对照组、模型组和黄芩苷组,用3.5%DSS诱导结肠炎模型,黄芩苷(30 mg/kg)干预7天,记录小鼠的疾病活动指数(DAI)评分,HE染色观察病理改变并评分,检测结肠髓过氧化物酶(MPO)活性,ELISA法检测肿瘤坏死因子(TNF)-α和白介素(IL)-6浓度,Real-time PCR法检测TLR2、TLR4和MyD88 mRNA表达水平。结果显示黄芩苷能有效抑制结肠炎小鼠DAI评分、结肠组织病理学评分和MPO活性,降低IL-6和TNF-α表达,降低TLR2、TLR4和MyD88 mRNA的表达。表明黄芩苷能有效缓解DSS诱导的结肠炎小鼠模型的症状,降低炎症反应,其作用机制可能是与TLRs/MyD88通路相关。The objective of this study was to elucidate whether baicalin reduces colonic inflammation in a mice colitis model through inhibition of the TLRs/MyD88 signaling pathway. C..57BL/6 mice were randomized into control group, DSS group （administered with 3.5% DSS solution） and baicalin group （ administered with 3.5% DSS solution and orally administered with baicalin,30 mg/kg body weight）. The disease activity index （DAI） score and histological score were evaluated. Myeloperoxidase activity was detected and colonic mucesal histological changes were observed by HE staning. The protein expression levels of Tttmomecrosis factor-or （TNF-a） and interleukin-6 （IL-6） levels in colonic tissue were determined by ELISA. The mRNA expression levels of TLR2 ,TLR4 and MyD88 in colonic tissue were detected by Real-time PCR. Compared with the DSS group, the baicalin-treatod group showed significant decreases in DAI, histological score,myeloperoxidase activity, expressions levels of TNF-a protein, IL-6 protein, TLR2 mRNA, TLR4 mRNA and MyD88 mRNA. Collectively, these findings highlighted that baicalin had significant therapeutic effects on DSS-induced colitis and the anti-inflammatory actions of baicalin may involve inhibition of the TLRs/MyD88 signaling pathway.

关 键 词：黄芩苷 炎症性肠病 TLRS MyD88通路 

分 类 号：R965.1[医药卫生—药理学]