鲜乳中常见污染细菌的分离与鉴定  

Isolation and identification of common bacterial contamination in fresh milk and bulging bags milk

作　　者：刘艳艳[1,2] 卢士英[2] 任洪林[2] 崔成[2] 盖冬雪 丁燕霞[2] 柳增善[2] 孟宪梅[1,2] 

机构地区：[1]吉林工商学院粮油食品深加工吉林省高校重点实验室,长春130062 [2]吉林大学动物医学学院人兽共患病研究教育部重点实验室,长春130062

出　　处：《中国乳品工业》2014年第6期16-18,33,共4页China Dairy Industry

基　　金：吉林省科技发展计划项目(201205054);粮油食品深加工吉林省高校重点实验室开放资金项目(2012008);吉林省世行贷款农产品质量安全项目(2011-Y36)

摘　　要：对吉林省污染鲜乳的细菌进行分离鉴定,以分析本地区鲜乳污染情况,为建立针对性检测鲜乳中的污染细菌提供参考。采集吉林省部分地区奶牛养殖场及农户散养奶牛自产鲜乳,采用常规培养方法进行分离培养。用聚合酶链式反应(PCR)扩增细菌16S rDNA,对PCR产物测序、分析,与Genbank上已知的细菌16S rDNA序列对比同源性,交叉同源性较高的细菌进行生化实验加以补充鉴定。结果表明,分离并鉴定出吉林省污染鲜乳的常见细菌,为鲜乳在生产加工过程中有针对性的检测特异性细菌奠定了基础。To isolate and identify the bacteria from fresh milk in Jilin Province so as to analyze the pollution situation of the fresh milk in the region. Collect fresh milk from some dairy tank and scattered cows in Jinlin Province. The 16SrDNA of the contaminated bacteria were amplified by polymerase chain reaction （PCk）. The PCR. products were sequenced and compared with the known sequences on the Genbank . Bacteria of the high homologous need to be supplemented with biochemical experiments. This study identified and isolated the contaminated f bacteria from fresh milk, which laid a foundation of detection the special bacteria in production and processing of fresh milk.

关 键 词：鲜乳 分离鉴定 常规培养 16S RDNA 聚合酶链式反应 

分 类 号：Q93-331[生物学—微生物学]