p38MAPK与ERK1/2在非小细胞肺癌中的表达及临床意义  

Expression of phospho-MAPK in non-small cell cancer and its clinicopathological significance

作　　者：邹俊勇[1,2] 陆国华[1] 周建英[1] 周建娅[1] 

机构地区：[1]浙江大学医学院附属第一医院呼吸内科,杭州310003 [2]宁波市第二医院

出　　处：《浙江医学》2014年第10期836-839,共4页Zhejiang Medical Journal

基　　金：国家自然科学基金资助项目(81101768);浙江省科技厅重大科技项目(2012C13G2010111)

摘　　要：目的探讨丝裂原活化蛋白激酶(MAPK)家族成员p38MAPK、细胞外信息调节蛋白激酶(ERK1/2)在非小细胞肺癌(NSCLC)中的表达及临床意义。方法选取43例NSCLC的肿瘤组织及其癌旁正常组织作为对照。采用蛋白免疫印迹法检测p38MAPK、ERK1/2的表达情况.分析其与临床特征(包括性别、年龄、肿块大小、淋巴结转移情况、分化程度、组织类型以及病理分期)的关系,及其表达程度与预后的关系。结果与配对的正常肺组织比较,phospho-p38MAPK、phospho-ERK1/2在NSCLC组织中活化上调(均P<0.01)。43例患者中37例phospho-p38MAPK的表达平均增长2.95倍。39例phospho-ERK1/2表达平均增长3.07倍.差异均有统计学意义(均P<0.01)。ERK1/2的表达与各临床特征均无明显相关(均P>0.05),而p38MAPK的活化与肿瘤大小以及年龄相关(均P<0.01)。ERK1/2未活化组、中度活化组与高度活化组的生存曲线依次下移,p38MAPK高度活化组生存曲线明显降低。Cox比例风险回归模型分析显示,phospho-p38MAPK的高表达是预后不良的预测因素(P<0.05)。结论 p38MAPK与ERK1/2的活化表达可能在NSCLC的发生/发展过程中具有重要意义,其中p38MAPK的活化程度可辅助作为非小细胞肺癌的预后评估因素。Objective To examine the expression of phospho- p38MAPK, phospho- JNK, and phospho- ERK1/2 in non- smal celllung cancer （NSCLC） and its clinicopathological significance. Methods Forty- three specimens of primary non- smal celllung cancer and paired normal lung tissue were collected. The expressions of phospho- p38MAPK, phos-pho- ERK1/2 and phospho- JNK were detected with Western- blot analysis. The association between clinicopathological parame-ters and the expression of phospho- p38MAPK and phospho- ERK1/2 protein was analyzed. Results Compared with corre-sponding normal lung tissue, the expression of phospho- p38MAPK and phospho- ERK1/2 in NSCLC tissue were increased （P〈0.01）. In 37 cases, phospho- p38MAPK expression were increased by average 2.95 times （P〈0.01）, while in 39 cases phos-pho- ERK1/2 expression were increased by 3.07 times （P〈0.01）. The expression of ERK1/2 was not correlated with clinicpatho-logical parameters （P〉0.05）. The activation of p38MAPK was correlated with tumor size and ages of patients （both P〈0.01）. The expressions of ERK1/2 and p38MAPK were negatively correlated with survival. Multivariate analysis revealed that the expres-sion of phospho- p38MAPK was an independent prognostic indicator for overal survival of patients （P=0.046）. Conclusion ERK1/2 and p38MAPK are activated in NSCLC, and the expression of phospho- p38MAPK can help to predict the prognosis of NSCLC.

关 键 词：丝裂原活化蛋白激酶 非小细胞肺癌 预后 

分 类 号：R734.2[医药卫生—肿瘤]