先天性心脏病儿童牙菌斑中白色念珠菌的携带情况  

Candida albicans carriage of children with congenital heart disease

作　　者：张琳[1] 黄晓旭[1] 赵玮[1] 

机构地区：[1]中山大学光华口腔医学院附属口腔医院.广东省口腔医学重点实验室,广东广州510055

出　　处：《临床口腔医学杂志》2014年第6期326-328,共3页Journal of Clinical Stomatology

基　　金：广东省自然科学基金(S2011020003247;8151008901000076);广东省科技计划基金(2010B050700004)

摘　　要：目的：研究先天性心脏病儿童牙菌斑中白色念珠菌的携带情况。方法：根据WHO龋齿诊断标准，以115名2～5岁的先心病儿童作为研究对象，其中有龋组86人，无龋组29人，同时选取115例健康儿童为对照组，临床检查记录龋失补牙数（dmft）、龋失补牙面数（dmfs）及可视菌斑指数（VPI）；采集龈上菌斑及龋坏组织，应用SYBR green I荧光定量PCR方法，对各组样本中白色念珠菌含量进行检测，所获得的数据进行统计学分析。结果：先心病组患龋率为74．8％，dmft、dmfs及VPI分别为5．69±5．69、11．83±15．14、43．8％±24．1％；对照组受检儿童患龋率为67．0％，dmft、dmfs、VPI分别为3．53±4．02、5．51±7．40、36．5％±24．1％。两组dmft，dmfs，VPI的差异有统计学意义。儿童牙菌斑中白色念珠菌的含量（Log10 copies/mL），先心病有龋组龋洞和牙颈部分别为6．71±2．92、5．64±3．11，无龋组为5．03±2．74；正常儿童有龋组龋洞和牙颈部分别为4．01±2．35、3．27±2．14，无龋组为3．43±2．56。先心病儿童与正常儿童牙菌斑中白色念珠菌含量差异具有统计学意义。结论：先心病儿童牙菌斑中白色念珠菌的含量高于正常儿童。口腔中的白色念珠菌，可能成为感染性心内膜炎的菌源之一，使先心病儿童成为感染心内膜炎的易感人群。Objective：To detect the levels of Candida albicans（C. albicans） in dental plaque samples in children with congenital heart disease（CHD） by fluorescent quantitative PCR. Method：According to the World Health Organization crite-ria in 2003,115 children with CHD aged 2 to 5 years were selected and classified into two groups：the caries-active group and the caries-free group. Real-time PCR based on the SYBR green I was used to quantities the levels of C. albicans. Re-sult：The caries prevalence rate,dmft,dmfs and visible plaque index （VPI） of the CHD group were 74.8 %,5.69 ± 5.69,11.83 ±15.14,43.8 %±24.1 %. In control group,the caries prevalence rate,dmft,dmfs and VPI were 67.0 %,3.53 ± 4.02,5.51±7.40 and 36.5 %±24.1 %.There were significant differences in dmft,dmfs and VPI score between the two groups. Log10 C.albicans counts of caries-active group and the caries-free group in children with CHD was 6.71 ±2.92、5.64 ±3.11 and 5.03 ±2.7 respectively. And log10 C.albicans counts of those in normal children was 4.01 ±2.35、3.27 ±2.14 and 3.43 ± 2.56. There were significant differences in C.albicans counts between the CHD group and the control group. Conclusion：The level of C. albicans in children with CHD was higher than that of normal children. C. albicans may be a bacteria source of IE and make children with CHD vulnerable to IE.

关 键 词：先天性心脏病 婴幼儿龋 白色念珠菌 感染性心内膜炎 

分 类 号：R788.1[医药卫生—口腔医学] R781.1[医药卫生—临床医学]