LIM矿化蛋白1在成骨分化前后人牙囊细胞中的表达  

Expression of LIM mineralization protein 1 after osteogenic differentiation of human dental follicle cells compared with undifferented

作　　者：常秀梅[1] 张克荣[1] 王书文[1] 齐香微 

机构地区：[1]广东医学院,广东东莞523808

出　　处：《临床口腔医学杂志》2014年第6期336-339,共4页Journal of Clinical Stomatology

基　　金：东莞市高等院校科研机构科技计划项目(2011108102028)

摘　　要：目的:研究人牙囊细胞成骨分化前后LIM矿化蛋白-1的表达。探讨LIM矿化蛋白-1在牙囊细胞向牙周组织分化过程中的作用。方法:组织块加酶消化法分离培养人牙囊细胞,实验组培养液中加入矿化诱导剂,对照组不加矿化诱导剂。培养4周后,免疫细胞化学检测骨涎蛋白的表达;Von Kossa染色检测矿化结节的形成。矿化诱导前后,采用RT-PCR技术检测LIM矿化蛋白-1。结果:培养4周,实验组矿化结节形成,骨涎蛋白免疫细胞化学呈阳性表达,Von Kossa染色阳性;对照组未形成矿化结节,骨涎蛋白表达弱阳性,Von Kossa染色阴性。RT-PCR结果显示LIM矿化蛋白-1在实验组强阳性表达,对照组弱表达。结论:LIM矿化蛋白-1与胚胎期人牙囊细胞的体外矿化过程相关,提示LIM矿化蛋白-1可能在胚胎期牙囊细胞的分化、矿化中发挥一定作用。Objective：To investigate Expression of LIM mineralization protein-1 after osteogenic differentiation of hu-man dental follicle cells compared with undifferented. Method：Human dental follicle cells were cultured in the presence of dexamethasone,asorbic acid and B-glycerophosphate for bone-like mineralized nodule formation ,characterized by Von Kos-sa staining and immunocyto chemistry of bone sialoprotein.Expression of LIM mineralization protein-1 was analzed by re-verse transcriptase-polymerase chain reaction. Result：LIM mineralization protein-1 were expressed both before mineraliza-tion and after,generally held at astatistically significantly higher level compared with the control. Conclusion：LIM mineral-ization protein-1 is found to be involved in the mineralization of human dental follicle cells.LIM mineralization protein-1 may play a role in the diferentiation and mineralization of human dental follicle cells.

关 键 词：牙囊细胞 分化 矿化 LIM矿化蛋白-1 

分 类 号：R786[医药卫生—口腔医学] R322[医药卫生—临床医学]