rhMG53蛋白对局灶性脑缺血再灌注损伤的保护作用研究  

Protective effect of rhMG53 protein on a focal cerebral ischemia/reperfusion in a rat model

作　　者：姚勇刚[1] 张猛[2] 李美香[1] 曾敬[1] 曾春雨[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所心血管内科重庆市心血管病研究所,重庆400042 [2]第三军医大学大坪医院神经内科重症监护室,重庆400042

出　　处：《解放军医学杂志》2014年第6期439-443,共5页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金重点项目(31130029);国家杰出青年科学基金(30925018)~~

摘　　要：目的观察重组人MG53蛋白对局灶性脑缺血再灌注(I/R)损伤的保护作用。方法采用大脑中动脉栓塞(MCAO)法建立SD大鼠脑IR模型,随机分为分假手术组、IR组、IR+rhMG53处理组(n=7),灌注24h后对各组大鼠进行神经系统Zea-Longa评分,然后处死大鼠,通过TTC染色观察脑梗死情况,HE染色观察大鼠脑组织病理改变。结果与IR组相比,IR+rhMG53处理组SD大鼠神经功能障碍明显改善,脑梗死面积缩小,脑组织坏死程度减轻,且保护作用与处理时间相关,外源性rhMG53对脑IR的有效保护时间窗在4h。结论外源性rhMG53对局灶性脑IR损伤具有明显的保护作用。Objective To identify the protective effect of rhMG53 （exogenous recombinant human MG53 protein） on focal cerebral ischemia/reperfusion injury in a rat model. Methods The cerebral ischemia reperfusion model was reproduced in SD rats using middle cerebral artery occlusion （MCAO） method, and the rats were then randomly divided into sham operation group, ischemia reperfusion group, and ischemia reperfusion＋rhMG53 group （n=7）. The Zea-Longa score of nervous system, brain tissue TTC staining and pathological sections were observed. Result Compared with the ischemia reperfusion group, nerve dysfunction was improved obviously in ischemia reperfusion＋rhMGS3 group. The area of cerebral infarction was reduced, and the extent of brain tissue necrosis was alleviated. Furthermorej the protective effect showed a relation with the treatment time. The time-window of effective protection of exogenous rhMG53 on cerebral ischemia reperfusion injury was within 4 hours. Conclusion The exogenous rhMG53 may have an effective protective effect on focal cerebral ischemia reperfusion injury.

关 键 词：脑梗塞 蛋白 MG53 再灌注损伤 

分 类 号：R743.33[医药卫生—神经病学与精神病学]