Survivin突变体对乳腺癌细胞的体内外作用  

Effects of Survivin-T34A Mutant on Breast Cancer Cell In Vitro and In Vivo

作　　者：邓晓慧[1] 宋海岩[1] 任铭新[1] 

机构地区：[1]河南新乡医学院人体解剖学教研室,新乡453003

出　　处：《生物医学工程学杂志》2014年第3期648-651,共4页Journal of Biomedical Engineering

基　　金：新乡医学院高学历人才科研启动项目;2011年新乡医学院重点研究领域招标课题项目

摘　　要：为了探讨Survivin负性突变体T34A对乳腺癌细胞MCF-7增殖与凋亡的影响,本文分别采用生理盐水、空载体质粒(PORF-9-null)及Survivin-T34A与MCF-7细胞共培养,观察体外MCF-7细胞的增殖及凋亡情况;建立乳腺癌裸鼠模型,体内实验检测Survivin-T34A对乳腺癌的抑制作用。结果表明转染了Survivin-T34A的细胞增殖率明显低于PORF-9-null组,电镜下观察可见细胞凋亡增多,流式细胞术检测显示其凋亡率明显高于空载体组。瘤内注射Survivin-T34A组的抑瘤效果明显,抑制率达47.1%。本研究提示Survivin-T34A负性突变体能够有效地抑制乳腺癌细胞的增殖,并促进其凋亡,从而发挥抗肿瘤作用。To study the effect of the proliferation and apoptosis of Survivin-T34A mutant on breast cancer MCF-7 cell, we adopted the method of cell culture in vitro to observe the proliferation and apoptosis of the cell. In the ex- periment, MCF-7 cells were randomly divided into three groups and transfected with normal saline, PORF-9-null and Survivin-T34A, respectively. Breast cancer nude mouse models were established to study anti-tumor effect of Sur- vivin-T34A in vivo. The activity of the cells in the Survivin-T34A-transfected group was lower than that in PORF-9- null group. The increase of cell apoptosis was observed under electron microscopy, meanwhile the apoptotic rate was obviously higher than that in PORF-9-null control by flow eytometry. Tumor inhibition effects of the mouse which received the injection of Survivin-T34A intratumoral injection were apparent, and the inhibition ratio was as high as 47. 1%. In conclusion, Survivin-T34A mutant has anti-tumor effect through efficiently inhibiting the growth of breast cancer MCF-7 cell and actively promoting apoptosis of cancer cells.

关 键 词：Survivin—T34A 突变体 乳腺癌 凋亡 

分 类 号：R737.9[医药卫生—肿瘤]