猪精子SPMI和Spermadhesins mRNA表达水平与精子活力的关联  

Association between Sperm Motility and Expression of SPMI and Spermadhesins Genesin Swine(Sus scrofa) Sperms

作　　者：高波[1] 陈才[1] 王赛赛[1] 薛松磊[1] 史云强[1] 钱跃[1] 沈丹[1] 宋成义[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009

出　　处：《农业生物技术学报》2014年第6期720-726,共7页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.31272406;No.31200920)

摘　　要：为了解析猪(Sus scrofa)精子中精子黏附蛋白基因家族(Spermadhesins)和精子运动抑制因子SPMI基因的表达与生物功能的关系,本研究采用精子上游法获得高低活力精子,结合体细胞标记基因分析检测所制备的精子RNA纯度,最后应用半定量RT-PCR技术分析高低活力精子mRNA表达差异。RT-PCR电泳显示,高活力精子中所有AQN1、AQN3、AWN(精子黏附蛋白基因家族)、PSPⅠ(猪精液蛋白1)、PSPⅡ(猪精液蛋白2)和SPMI的mRNA表达丰度均低于低活力精子。单因素方差分析显示,低活力精子组中AQN1、AQN3、AWN、PSPⅠ、PSPⅡ和SPMI mRNA相对表达水平均显著高于高活力精子组。研究结果提示Spermadhesins和SPMI基因在精子中的mRNA表达水平有可能作为精子活力的分子标记,从而为猪分子育种提供参考。To explore the biological functions of SPMI （sperm motility inhibitor） and Spermadhesins genes in swine（Sus scrofa） sperms, the high and low motility sperms were prepared by swim-up method. Sperm totalRNA was isolated by Trizol method. Somatic cells marker genes were detected to determine the RNA purity. Then SPMI and Spermadhesins mRNA level in high motility sperms was compared with low motility spermsby semiquantitative RT-PCR. The results showed that AQNI, AQN3, AWN （Spermadhesins）, PSP Ⅰ （porcine seminal protein Ⅰ ）, PSP Ⅱ （porcine seminal protein Ⅱ ） and SPMI mRNA level in high motility sperms weresignificantly lower than that in low motility sperms. The result of one-way ANOVA showed that the ralative expression level ofAQN1, AQN3, AWN, PSP Ⅰ, PSP Ⅱ and SPMI mRNA was significantly higher in lowmotility sperm than in high motility sperm. This result suggested that SPMI and Spermadhesins mRNA level in sperms could be as a molecular marker for sperm motility, and provide a reference for molecular breeding pig.

关 键 词：猪 SPMI Spermadhesins MRNA 精子活力 

分 类 号：S78[农业科学—林学]