过敏性哮喘患者外周血CD4^+T细胞表达CD39和CD73水平变化及其临床意义  

Expression of CD39 and CD73 by CD4^+T cells in peripheral blood of patients with allergic asthma and its clinical significance

作　　者：王林林[1,2] 王玺[3] 时国朝[1] 万欢英[1] 周敏[1] 侯小霞[1] 潘丽娜[1] 陶连琴[1] 石宝玉[1] 宋慧慧[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院呼吸科,上海200025 [2]第二军医大学附属长征医院内科基地,上海200003 [3]第二军医大学附属长征医院急救科,上海200003

出　　处：《内科理论与实践》2014年第2期130-133,共4页Journal of Internal Medicine Concepts & Practice

基　　金：国家自然科学基金项目(项目编号:81270083);上海市科委基础研究重点项目(项目编号:08JC1415200)

摘　　要：目的:初步探讨CD4+T细胞表达的腺苷CD39和CD73在过敏性哮喘患者外周血中的水平变化及与叉头蛋白3(FoxP3)、GATA结合蛋白3(GATA3)和维A酸相关孤儿受体(ROR)-γt mRNA相关性。方法:选择54例对屋尘螨过敏的慢性持续期哮喘患者[间歇状态和轻度持续哮喘组(间歇-轻度组)29例,中重度组25例]和45名健康志愿者,通过荧光实时定量PCR方法检测并比较研究对象外周血CD4+T细胞中CD39、CD73、FoxP3、GATA3和ROR-γt mRNA水平。结果:CD39 mRNA水平正常对照组明显高于间歇-轻度组(P=0.000),间歇-轻度组明显高于中重度组(P=0.001)。CD73 mRNA水平正常对照组明显高于间歇-轻度组(P=0.001),哮喘亚组间差异无统计学意义(P=0.126)。哮喘组CD4+T细胞表达的CD39 mRNA与FoxP3 mRNA水平呈显著正相关性(r=0.607,P=0.000),与GATA3 mRNA水平呈显著负相关性(r=-0.505,P=0.000),与ROR-γt mRNA无显著相关性(r=-0.287,P=0.054)。哮喘组CD4+T细胞表达的CD73 mRNA与FoxP3和ROR-γt mRNA水平无显著相关性(r=0.110,P=0.450;r=-0.227,P=0.138),与GATA3mRNA水平呈显著负相关性(r=-0.286,P=0.040)。结论:CD4+T细胞表达的CD39和CD73在过敏性哮喘免疫反应过程中起到重要作用。Objective To investigate the expression of CD39 and CD73 by CD4＋T cells in peripheral blood of patients with allergic asthma and its correlation with GATA binding protein （GATA）3, retinoid-ralated orphan receptor （ROR）-γt and forkhead box protein （FoxP）3 mRNA expressed by CD4＋T cells. Methods Fifty-four patients with chronic persistent allergic asthma （29 with intermittent-mild form, 25 with moderate-severe form） and 45 healthy volunteers were enrolled in this study. The expression of CD39, CD73, GATA3, ROR-γt and FoxP3 mRNA in CD4＋T cells was determined by fluorescent quantitative real-time PCR. Results The expression level of CD39 in CD4＋T cells in healthy controls （n=45） was higher than that in intermittent-mild asthma （n=29） （P=0.000）, and the level in intermittent-mild asthma was higher than that in moderate-severe asthma （n=25） （P=0.001）. The expression level of CD73 in CD4＋T cells in healthy controls was higher than that in intermittent-mild asthma （P=0.001）, and there was no significant difference between intermittent-mild asthma and moderate-severe asthma （P=0.126）. CD39 mRNA was positively correlated with FoxP3 mRNA （r=0.607, P=0.000）, and negatively correlated with GATA3 mRNA （r=-0.505, P=0.000）, and not correlated with ROR-γt mRNA （r=-0.287, P=0.054）. CD73 mRNA was not correlated with FoxP3 and ROR-γt mRNA （r=0.110,P=0.450; r=-0.227, P=0.138）, and was negatively correlated with GATA3 mRNA （r=-0.286, P=-0.040）. Conclusions CD39 and CD73 expressed by CD4＋T cells may play an important role in mediating the immune response in patients with allergic asthma.

关 键 词：过敏性哮喘 CD4+T细胞 CD39 CD73 

分 类 号：R562.25[医药卫生—呼吸系统]