GPER在子宫内膜样腺癌中的表达及其与ER的相关性  

Expression of GPER in endometrioid adenocarcinoma and correlation between GPER expression level and ER

作　　者：邰海凤[1] 陈姝彤[1] 欧阳一芹[1] 童晓文[1] 

机构地区：[1]同济大学附属同济医院,上海200065

出　　处：《现代妇产科进展》2014年第5期333-336,340,共5页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(No:81101967)

摘　　要：目的:研究GPER(G protein-coupled estrogen receptor)在子宫内膜样腺癌(EEC)组织中的表达情况,探讨GPER对EEC临床病理因素、预后的意义及其与雌激素受体(ER)之间的相关性。方法:采用实时定量PCR技术检测EEC组织中的GPER mRNA含量,免疫组化法检测GPER蛋白含量,分析GPER表达对EEC的临床意义。结果:GPER高表达于FIGO晚期、低分化、子宫肌层深浸润及淋巴转移的EEC组织中。单因素分析结果显示,GPER mRNA高表达的患者预后较差(OS,P=0.001;DSS,P=0.001;PFS,P<0.001),GPER蛋白阳性的患者预后亦较差(OS,P=0.009;DSS,P=0.009;PFS,P<0.001)。多因素分析发现,GPER不是影响EEC患者预后的独立因子。GPER与ER无统计学相关性。结论:GPER不是EEC预后的独立因子,但在其临床生物学行为中扮演重要的角色。Objective：To evaluate the relationships of G protein-coupled estrogen receptor （GPER） with estrogen receptor of and clinical outcome of endometrioid endometrial adenocarcinoma patients. Methods： GPER mRNA was detected with real-time PCR, and GPER protein was detected with immunohistochemistry. Results were correlated with clinicopathological predictors of adverse outcome and survival. Results ： GPER was found over-expressed in patients with high grade, advanced stage, deep myometrial invasion and lymph node involvement, either in levels of protein or mRNA. In univariate analyses, higher levels of mRNA and protein of GPRE were correlated to a poorer overall survival （P=0. 001 ）,disease-specific survival （P =0. 001 ） and progression-free survival （P〈0. 001 ,P〈0. 001 ）. Yet multivariate analyses confirmed GPER expression was not an independent prognostic factor for patient survival. GPER had no correlation with ER. Conclusions： GPER is not an independent influence factor for EEC. GPER could be an interesting factor in clinical biological behavior of EEC.

关 键 词：子宫内膜样腺癌 GPER 生存率 雌激素受体 

分 类 号：R737.33[医药卫生—肿瘤]