胎鼠胰腺干细胞与胰岛联合移植保护胰岛  

Co-transplantation of fetal rat pancreatic stem cells and islets of langerhans to preserve viability of islets

作　　者：张育森[1] 鲍世韵[1] 张悦[1] 李富荣[2] 

机构地区：[1]暨南大学第二临床医学院(深圳市人民医院)肝胆胰外科,广东省深圳市518020 [2]暨南大学第二临床医学院(深圳市人民医院)临床研究中心,广东省深圳市518020

出　　处：《中国组织工程研究》2014年第19期3054-3059,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:胰腺干细胞可在体外维持胰岛的结构,减少胰岛细胞坏死及凋亡,延长胰岛的体外存活时间,保护胰岛的活性。目的:探索胎鼠胰腺干细胞与胰岛共移植体内保护移植胰岛,提高胰岛移植疗效的可行性。方法:将成年大鼠35只随机等分为联合移植组、单独胰岛移植组、单纯胰腺干细胞移植组、模型组及对照组,前4组均腹腔注射链脲佐菌素-柠檬酸盐缓冲液建立糖尿病模型。联合移植组、单独胰岛移植组、单纯胰腺干细胞大鼠分别在左侧肾包膜下移植分离纯化孕16 d SD大鼠胎鼠胰腺干细胞和/或成年SD大鼠胰岛。结果与结论:联合移植组大鼠移植后5 d内血糖可降至正常,血浆胰岛素达到正常水平,胰岛存活时间(18.2±2.4)d;单独移植组大鼠血糖可于移植后1周内降至正常,胰岛存活时间(14.4±2.1)d;两组胰岛存活时间比较差异有显著性意义(P<0.05)。而其他组糖尿病大鼠血糖均未能降至正常范围。说明胎鼠胰腺干细胞与胰岛共移植可延长胰岛体内存活时间,保护胰岛功能,提高移植疗效。BACKGROUND：Pancreatic stem cels can maintain the islet structurein vitro, reduce necrosis and apoptosis of islet cels,in vitro prolong islet survival, and keep islet activity. OBJECTIVE： To observe the possibility of preserving viability of isletsin vivo by co-transplanting fetal rat pancreatic stem cels and islets so as to improve the outcome of islet transplantation. METHODS：Thirty-five adult rats were randomly divided into five groups, including co-transplantation, islet transplantation alone, pancreatic stem celltransplantation alone, diabetes control and normal control groups. Diabetic models were established in the former four groups by intraperitoneal injection of streptozotocin-citrate buffer. Pancreatic stem cels from the fetal rats at pregnant 16 days or islets from adult Sprague-Dawley rats. RESULTS AND CONCLUSION：In the co-transplantation group, the levels of blood glucose and plasma insulin returned to the normal after 5 days of co-transplantation, and the survival time of islets was （18.2±2.4） days. In the islet transplantation alone group, the level of blood glucose was reduced to normal after 1 week of transplantation, and the survival time of islets was （14.4±2.1） days. Significant different was found between the survival time of islets between this two groups （P 〈 0.05）. However, the level of blood glucose was stil abnormal in the other groups. These findings indicate that the co-transplantation of fetal rat pancreatic stem cels and islets can prolong the survival time of isletsin vivo, protect viability of islets and improve the outcome of islet transplantation.

关 键 词：干细胞 移植 胎鼠 胰腺干细胞 胰岛 胰岛移植 联合移植 糖尿病 

分 类 号：R394.2[医药卫生—医学遗传学]