帕金森病肠功能分析及CXCR4在肠神经系统中的表达  

Intestinal function analysis in Parkinson's disease and the expression of CXCR4 in the enteric nervous system

作　　者：张静[1] 暴丽华[1,2] 吴金涛[1] 李贵宝[1] 刘海莉[1] 岳庆伟[1] 朱德晓[1] 孙东[1] 宋守阳 丁兆习[1] 孙晋浩[1] 

机构地区：[1]山东大学医学院人体解剖学教研室,山东济南250012 [2]长治市人民医院神经内科,山西长治046000

出　　处：《山东大学学报（医学版）》2014年第5期25-29,共5页Journal of Shandong University:Health Sciences

基　　金：教育部博士点基金(20120131120049);山东省自然科学基金(ZR2012HQ018;ZR2010HM051);山东省科技发展计划(2011GSF11810);山东大学自主创新基金(2012TS113)

摘　　要：目的研究帕金森病(PD)大鼠肠功能的改变,探讨趋化因子CXCL12受体CXCR4在肠神经系统中的功能及作用机制。方法将20只SD大鼠随机分为6-OHDA组和对照组。6-OHDA组经6-OHDA脑内定点注射建立PD动物模型,建模4周后收集两组大鼠1 h粪便排出量,计算粪便含水量。利用免疫荧光染色法检测胃肠神经细胞CXCR4表达的变化。结果与对照组相比,6-OHDA组大鼠1 h粪便排出量及含水量明显降低(P<0.01),而且胃肠神经组织中CXCR4阳性细胞数量明显减少,但荧光强度显著增强(P<0.01)。结论 PD大鼠胃肠神经系统中CXCR4表达水平降低与其胃肠功能障碍密切相关。Objective To investigate the changes of intestinal function in rats with Parkinson’s disease（PD） and explore the function and mechanism of CXCL12/CXCR4 in enteric nervous systems. Methods Twenty SD rats were randomly divided into 6-OHDA group and control group. In 6-OHDA group,SD rats were treated with 6-OHDA to establish PD model. Four weeks later,the one-hour fecal excretion and water content of the feces in two groups were measured. In addition,the expression of CXCR4 in the gastrointestinal nerve plexus was detected by immunofluorescent staining. Results Compared with those in the control group,the one-hour fecal excretion and water content of the feces in 6-OHDA group were significantly decreased（P ＆lt; 0. 01）. In 6-OHDA group,the number of CXCR4-positive cells in gastrointestinal nerve plexus was obviously decreased while the fluorescence intensity was increased（P ＆lt; 0. 01）. Conclusion Gastrointestinal dysfunction of PD rats may be related to the decreasing expression of CXCR4 in the enteric nervous systems.

关 键 词：帕金森病 肠神经系统 6-OHDA CXCR4 

分 类 号：R742.5[医药卫生—神经病学与精神病学]