罗勒多糖对卵巢癌SKOV3细胞VEGF受体表达的调控作用及对其侵袭能力的影响  

Regulation of vascular endothelial growth factor receptors expression in SKOV3 cells by basil polysaccharide and its effect on SKOV3 cell invasion

作　　者：付伟[1] 高文娟[2] 张志勉[1] 曲迅[2] 赵玉霞[3] 

机构地区：[1]山东大学齐鲁医院查体中心,山东济南250012 [2]山东大学齐鲁医院临床基础研究所,山东济南250012 [3]山东大学齐鲁医院中医科,山东济南250012

出　　处：《山东大学学报（医学版）》2014年第5期39-43,48,共6页Journal of Shandong University:Health Sciences

摘　　要：目的探讨罗勒多糖(BPS)对卵巢癌SKOV3细胞血管内皮生长因子(VEGF)受体表达的调控作用及对其侵袭能力的影响。方法酶联免疫吸附试验(ELISA)、实时定量PCR及流式细胞术分别检测BPS对SKOV3细胞分泌VEGF的影响及对VEGF受体(VEGFRs)在SKOV3细胞表达的调控作用;Transwell试验检测不同浓度BPS对SKOV3细胞侵袭能力的影响;通过特异性shRNA慢病毒表达载体感染的方法稳定敲减VEGFR2在SKOV3细胞的表达后,Transw ell试验检测其对BPS侵袭抑制能力的影响。结果 BPS并未影响SKOV3细胞VEGF分泌水平的表达,对VEGFR1、VEGFR3在SKOV3的表达也无显著影响,但可明显上调VEGFR2在SKOV3细胞的表达;Transwell试验显示BPS可抑制SKOV3细胞的体外侵袭能力,并具有一定的浓度依赖性;成功感染携带VEGFR2 shRNA的慢病毒表达载体后,VEGFR2在SKOV3细胞的表达显著下调(P<0.01),且BPS对敲减VEGFR2表达的SKOV3细胞侵袭抑制能力显著降低。结论 BPS可显著抑制SKOV3细胞的侵袭能力,其抑制作用部分是通过上调VEGFR2在SKOV3细胞的表达介导的。Objective To investigate the effect of basil polysaccharide（BPS） on the expression of vascular endothelial growth factor receptors（VEGFRs） in SKOV3 ovarian cancer cells and its involvement in regulating the invasion of SKOV3 cells. Methods Vascular endothelial growth factor（VEGF） secretion by SKOV3 cells after BPS treatment was analyzed by ELISA and the regulation of VEGFRs in SKOV3 cells by BPS was assessed by qRT-PCR and flowcytometry. Transwell assay was performed to investigate the effect of different concentrations of BPS on SKOV3 cell invasion. Lentiviral shRNA expression vector infection was utilized to stably knockdown the expression of VEGFR2 in SKOV3 cells and the impact of BPS on VEGFR2-knockdown SKOV3 cells was further analyzed by Transwell assay. Results Secretion of VEGF from SKOV3 cells was not altered by BPS treatment,and BPS showed no significant effect on VEGFR1 and VEGFR3 expressions in SKOV3 cells. However,VEGFR2 expression in SKOV3 cells was remarkably increased by BPS treatment both in the mRNA level and surface protein level. Transwell assay showed BPS significantly inhibited the invasion of SKOV3 cells in vitro in a concentration-dependent manner. SKOV3 cells infected with specific VEGFR2 shRNA lentiviral vector showed decreased expression of VEGFR2,and the inhibitory effect of BPS on VEGFR2-knockdown SKOV3 cells was lower than that on the negative control cells. Conclusion BPS significantly inhibited the invasion of SKOV3 cells in vitro,which was partially mediated by the up-regulation of VEGFR2 in SKOV3 cells.

关 键 词：卵巢肿瘤 罗勒多糖 侵袭 血管内皮生长因子受体2 

分 类 号：R737.31[医药卫生—肿瘤]