神经生长因子前体在病理性瘢痕中的表达  

Expression of nerve growth factor precursor in pathological scars

作　　者：周一冲[1] 张芮 冯永强[1] 冯璋[1] 王一兵[1] 

机构地区：[1]山东大学附属省立医院烧伤整形科,山东济南250021 [2]山东医学高等专科学校病理与病理生理教研室,山东济南250002

出　　处：《山东大学学报（医学版）》2014年第5期92-95,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81171818);济南科技局课题(201102074)

摘　　要：目的观察神经生长因子前体(proNGF)在病理性瘢痕中的表达。方法收集增生性瘢痕23例和瘢痕疙瘩20例作为病理性瘢痕组,正常皮肤20例作为对照组,采用免疫组织化学技术检测proNGF在3种组织中的表达和分布,Western blotting检测3种组织中proNGF蛋白表达量的差异。结果 proNGF在正常皮肤、增生性瘢痕和瘢痕疙瘩中的表达均呈阳性,表达位于3种组织的表皮和成纤维细胞,免疫组化结果显示,增生性瘢痕和瘢痕疙瘩中proNGF的阳性表达均低于正常皮肤组织(χ2=8.23,P<0.05;χ2=8.29,P<0.05),Western blotting结果同样显示增生性瘢痕和瘢痕疙瘩中proNGF的蛋白表达量低于正常皮肤组织(t=14.02,P<0.01;t=20.80,P<0.01)。结论病理性瘢痕中proNGF的含量低于正常皮肤,病理性瘢痕组织的增生性可能与proNGF的低表达有关。Objective To observe the expression of nerve growth factor precursor（proNGF） in pathological scars. Methods A total of 23 hyperplastic scar samples and 20 keloid samples（pathological scar group） and 20 normal skin tissues（control group） were collected. The proNGF expressions were examined with immunohistochemical and Western blotting techniques. Results The proNGF was positively expressed in all three tissue types,especially in epidermal cells and fibroblasts. Immunohistochemical examination indicated that proNGF expression was significantly lower in hyperplastic scars and keloids than in normal skin（χ2= 8. 23,P ＆lt; 0. 05; χ2= 8. 29,P ＆lt; 0. 05）. Western blotting showed that protein expression of proNGF was markedly lower in hyperplastic scars and keloids than in normal skin（t = 14. 02, P ＆lt; 0. 01; t = 20. 80,P ＆lt; 0. 01）. Conclusion Compared with normal skin,proNGF expression in pathological scars is significantly lower,suggesting the negative relationship between proNGF expression and scar proliferation.

关 键 词：神经生长因子前体 病理性瘢痕 皮肤 成纤维细胞 

分 类 号：R364.3[医药卫生—病理学]