七叶一枝花挥发油成分及其抑菌活性分析  

Analysis of Chemical Components of Volatile Oil from Paris polyphylla and Their Antibacterial Activities

作　　者：刘志雄[1,2] 刘祝祥[1,3] 田启建[1,3] 

机构地区：[1]吉首大学植物资源保护与利用湖南省高校重点实验室,湖南吉首416000 [2]吉首大学物理与机电工程学院材料系,湖南吉首416000 [3]吉首大学生物资源与环境科学学院,湖南吉首416000

出　　处：《中药材》2014年第4期612-616,共5页Journal of Chinese Medicinal Materials

基　　金：湖南省教育厅重点项目(11A099);植物资源与利用湖南省重点实验室开放基金项目(JSK201202);吉首大学博士学成返校科研基金项目(jsdxxcfxbskyxm201205);湖南省重点学科吉首大学生态学学科建设项目(JSU071301);吉首大学新开课程建设项目(2012KCB05)

摘　　要：目的:分析七叶一枝花挥发油化学成分并检测其抑菌活性。方法:采用气相色谱-质谱联用(GC-MS)技术对超临界CO2提取的七叶一枝花挥发油进行成分分析,并采用滤纸片法检测该挥发油对5种常见病原菌的抑菌活性。结果:从七叶一枝花挥发油中鉴定了75个化合物,所鉴定成分占挥发油色谱总峰面积的98.46%,其主要成分为:邻苯二甲酸-异丁基-3-戊烯基酯、9,12-十八碳-二烯酸、八氢-4α-甲基-7-异丙基-2-(1H)-萘酮、甘油、油酸甲酯、1,2-邻苯二甲酸-丁基-2-异丁基酯、邻苯二甲酸二(2-乙基己基)酯、2-十四烷基-甘氨酰胺、(9,12)-十八碳二烯烷氧基乙醇、棕榈酸-2-羟基-1-(羟甲基)乙酯、10-甲基月桂酸甲酯、甘油醚和3,3-二乙氧基-1-丙醇;七叶一枝花挥发油对藤黄微球菌、产气杆菌、枯草芽孢菌有明显的抑制作用,对大肠杆菌和变形杆菌抑制作用较差。结论:该实验结果为七叶一枝花的进一步研究和开发应用提供理论依据。Objective： To analyze the chemical components of volatile oil from Paris polyphylla by GC-MS,and to investigate their antimicrobial activities.Methods： The chemical compositions of essential oil,which from Paris polyphylla obtained by supercritical carbon dioxide,were analyzed by GC-MS,and their antibacterial properties for five common pathogens were screened by filter paper method.Results： The components in the volatile oils were analyzed by GC-MS,and 75 compounds comprising for 98.46% of the essential oil were identified.The major components of essential oil were phthalic acid-isobutyl-hex-3-enyl ester,9,12-octadecadienoic acid,2（ 1H）-naphthalenone,octahydro-4α-methyl-7-（ 1-methylethyl）-,glycerin,9-octadecenoic acid-methyl ester,1,2-benzenedicarboxylic acid-butyl-2-methylpropyl ester,bis（ 2-ethylhexyl） phthalate,2-myristynoyl-glycinamide,ethanol,2-（ 9,12）-octadecadienyloxy,hexadecanoic acid,2-hydroxy-1-（ hydroxymethyl） ethyl ester,dodecanoic acid,10-methyl,methyl ester,diglycerol and 3,3-diethoxy-1-propanol; The bacteriostatic experiment results indicated that this volatile oil had strong inhibitory effects on Micrococcus,Aerobacter and Brevibacterium,whereas it had poor effects Escherichia coli and Proteus vulgaris.Conclusion： The experiment provides a scientific basis for further development and utilization of Paris polyphylla.

关 键 词：七叶一枝花 挥发油 超临界CO2提取 GC-MS分析 抑菌活性 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]