胃癌组织原发灶及淋巴结转移灶中E-cadherin、β-catenin蛋白表达及意义  

Significance of E-cadherin, β-catenin expression in the primary gastric carcinomas versus lymph nodal metastases

作　　者：王媛媛[1] 詹晓芬[1] 李进展[2] 沈金辉[1] 王少洪[1] 

机构地区：[1]汕头市中心医院病理科,515041 [2]汕头市中心医院胃肠外科,515041

出　　处：《国际医药卫生导报》2014年第13期1862-1866,共5页International Medicine and Health Guidance News

基　　金：2010年汕头市科技计划项目（201063-2）

摘　　要：目的 探讨E-钙粘蛋白（E-cadherin,E-cad）、β-连环素（β-catenint, β-cat）在胃腺癌原发灶及淋巴结转移灶中的表达,研究其与临床病理诸因素的关系,并探讨二者对淋巴结转移的意义.方法 采用免疫组化方法检测50例胃癌组织及其淋巴结转移灶、20例正常胃粘膜组织中E-cad、β-cat的表达并分析其与临床诸因素的关系.结果 E-cad和β-cat在胃癌组织中的表达率分别为40％（20/50）、36％（18/50）低于正常胃粘膜组的表达率100％（20/20）、100％（20/20）.中低分化组19.23％（5/26）、19.23％（5/26）,侵及浆膜层组30.77％（12/39）、30.77％（12/39）,有淋巴结转移组的表达27.27％（9/33）、30.30％（10/33）,分别低于高分化组75％（6/8）、75％（6/8）未侵及浆膜组72.72％（8/11）、81.81％ （9/11）及无淋巴结转移组64.70％（11/17）、70.59％（12/17）,差异有统计学意义（P＜0.01）.E-cad和β--cat的表达在原发灶及转移灶的表达均呈正相关（r=0.836,P＜ 0.01）,E-cad和β-cat在淋巴结原发灶及转移灶中的表达差别无统计学意义（P＞0.05）.结论 E-cad、β-cat在胃癌组织中的表达与肿瘤的浸润和转移有关,联合检测E-cad、β--cat有可能成为判断胃癌恶性程度和预后的重要指标.检测淋巴结转移灶对无法获得原发灶的患者推测预后有较高的参考价值,或者通过术前活检可以预测患者淋巴结转移情况.Objective To investigate the expressions of protein E-cad,β-catenin in the primary gastric carcinomas versus lymph nodal metastases,determine their clinical pathologic features and prognosis.Methods Immunohistochemical method was used to determine the expression of E-cad,β-cat in 50 gastric carcinoma specimens and 20 specimens of normal gastric tissues,and analyze their correlation with the clinical pathologic characteristics.Results The positive expression rates of E-cad and β-cat were 40％ （20/50） and 36％ （18/50） respectively,which were all lower than those in normal gastric tissues [100％ （20/20）,100％ （20/20）,all P ＜ 0.01],respectively.The positive expression rates of E-cad and β-cat were significantly lower in the moderate-low differentiation group 19.23％ （5/26）,19.23％ （5/26）,infiltration into serosa coat group 30.77％ （12/39）,30.77％ （12/39）,lymphatic metastasis group 27.27％ （9/33）,30.30％ （10/33） than in the well-differentiated gastric group75％ （6/8）,75％ （6/8）,no infiltration into serosa coat group 72.72％ （8/11）,81.81％ （9/11）,non-metastatic group 64.70％ （11/17）,70.59％ （12/17）,（P ＜ 0.01）.E-cad expression was positively correlated with β-cat in gastric carcinoma （r=0.836,P ＜ 0.01）.The expression of E-cad and β-cat showed no statistical significant difference between primary gastric carcinomas and lymph nodal metastases （P ＞ 0.05）.Conclusion The positive expression of E-cad and β-cat suggest their correlation with infiltration and development of gastric carcinoma.E-cad and β-cat can be used as valuable molecular markers for progression and prognosis of gastric carcinoma.To detect lymph nodal is valuble if we can not get primary carcinomas.Preoperative biopsy can predicts the lymph node status.

关 键 词：上皮型钙黏素 Β-连环素 胃肿瘤 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]