高尔基体膜蛋白73在原发性肝癌患者血清中的表达及其早期诊断价值  

The expression level of serum Golgi protein- 73 in primary hepatocellular carcinoma and its clinical value in making early diagnosis

作　　者：陈海波[1] 顾玉明[1] 王洵[1] 

机构地区：[1]徐州医学院附属医院介入放射科

出　　处：《介入放射学杂志》2014年第6期528-531,共4页Journal of Interventional Radiology

摘　　要：目的探讨原发性肝癌患者血清中高尔基体膜蛋白-73(Golgi protein-73,GP73)的表达水平及其早期诊断价值。方法收集人外周血血清490例,应用酶联免疫定量测定法和电化学发光法分别检测血清中GP73表达和AFP水平。结果肝细胞肝癌、胆管细胞癌及布加综合征合并肝细胞肝癌患者血清GP73的表达水平均高于其他各组(P<0.05);GP73、AFP诊断肝细胞肝癌(包括布加综合征合并肝细胞肝癌)的受试者工作特征曲线下面积、灵敏度、特异度、正确率及最佳临界值分别为0.868、90.8%、75.5%、77.6%、43.40 ng/ml;0.739、60%、83.2%、80.2%、20 ng/ml。GP73诊断肝细胞肝癌的受试者工作特征曲线下面积及灵敏度显著高于AFP(P<0.05);特异度及正确率差别无统计学意义(P>0.05)。GP73诊断胆管细胞癌的受试者工作特征曲线下面积为0.774,灵敏度、特异度及正确率分别为88.6%、72.7%及73.9%,最佳临界值为45.40 ng/ml。结论血清GP73对原发性肝癌的诊断价值优于AFP,有望成为原发性肝癌早期诊断的血清标记物。Objective To investigate the expression level of serum Golgi protein-73 （GP73） in patients with primary hepatocellular carcinoma （HCC）, or accompanied with Budd-Chiari syndrome （BCS）, and to discuss its clinical value in making early diagnosis. Methods The peripheral serum samples were collected in 490 individuals, including patients with HCC （n = 65）, cholangiocarcinoma （CC, n = 35）, Budd-Chiari syndrome accompanied with HCC （n = 40）, type B virus hepatitis （n = 60）, cirrhosis after B hepatitis （n = 60）, Budd-Chiari syndrome complicated by cirrhosis （n = 60）, other malignant lesions （n =50）, benign hepatic lesions （n = 60） and healthy volunteers （n = 60）. The expression levels of serum GP73 and AFP were determined by using Elisa and electrochemiluminescence methods separately. Results The expression level of serum GP73 in the patients with HCC , CC or BCS plus HCC were significantly higher than that in the other individuals （P 0.05）. The area under ROC, sensitivity, specificity and the accuracy of GP73 for the diagnosis of CC were 0.774 , 88.6%, 72.7% and 73.9%respectively, and the optimal cut-off value was 45.40 ng/ml. Conclusion In diagnosing HCC, the expression level of serum GP73 is superior to AFP. It is hopeful that serum GP73 may be proved to be an early serum tumor marker for HCC.

关 键 词：原发性肝癌 高尔基体膜蛋白-73 甲胎蛋白 诊断价值 

分 类 号：R735.7[医药卫生—肿瘤]