子宫颈上皮内瘤变及鳞状细胞癌中Rb、MCM7的表达及临床意义  

Expression and significance of Rb,MCM7 proteins in cervical intraepithelial neoplasia and squamous cell carcinoma

作　　者：林海瑞 童丹[1] 宋文静[1] 

机构地区：[1]天津医科大学病理学教研室,天津300070 [2]天津医科大学临床医学院,天津300070

出　　处：《临床与实验病理学杂志》2014年第6期633-636,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨视网膜母细胞瘤(retinoblastoma,Rb)易感基因、微小染色体维持蛋白7(minichromosome maintenance proteins7,MCM7)在子宫颈上皮内瘤变(cervical intraepithelial neoplasias,CIN)及鳞状细胞癌中的表达及意义。方法应用免疫组化PV 6000两步法检测Rb、MCM7在10例正常子宫颈组织、90例CIN组织(其中CIN1、CIN2和CIN3各30例)、30例子宫颈鳞状细胞癌组织中的表达,并分析其表达水平的变化。结果 Rb在正常子宫颈、CIN和子宫颈鳞状细胞癌组织中表达依次降低,组间差异有统计学意义(H=54.140,P<0.001);MCM7在正常子宫颈、CIN和子宫颈鳞状细胞癌组织中表达依次增高,组间差异有统计学意义(H=66.751,P<0.001);Rb与MCM7的表达呈负相关(rs=-0.536,P<0.001)。结论 Rb、MCM7表达与CIN病变级别及子宫颈鳞状细胞癌相关,提示Rb、MCM7可能参与CIN和子宫颈鳞状细胞癌的发生、发展。Purpose To investigate the expression of retinoblastoma （ Rb）, minichromosome maintenance proteins 7 （ MCM7 ） proteins and its significance in cervical intraepithelial neoplasia and squamous cell carcinoma. Methods Experimental specimens were collected as follows ： 10 cases of normal cervical tissue, 90 cases of cervical intraepithelial neoplasias （including 30 cases of CIN1,30 cases of CIN2 and 30 cases of CIN3） and 30 cases of cervical squamous cell carcinoma, the expression of Rb, MCM7 were examined by immunohistochemical （PV 6000 two-step method） technique. Results The expression degree of Rb reduced gradually from normal cervical tissue, cervical intraepithelial neoplasia and cervical squamous cell carcinoma （H = 54. 140, P 〈 0. 001 ）. The expression degree of MCM7 increased gradually from normal cervical tissue, cervical intraepithelial neoplasia and cervical squamous cell carcinoma （ H = 66.751, P 〈 0. 001 ）. The expression level of Rb was negatively correlated with MCM7 （rs = - 0. 536, P 〈 0. 01 ）. Conclusion The expression of Rb, MCM7 proteins is associated with the level of cervical intraepithelial neoplasia and cervical squamous cell carcinoma, and they probably play an important role in the process of cervical intraepithelial neoplasia and cervical squamous cell carcinoma.

关 键 词：子宫颈肿瘤 鳞状细胞癌 子宫颈上皮内瘤变 视网膜母细胞瘤易感基因 微小染色体维持蛋白7 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]