纳米二氧化钛对幼年和成年大鼠肝、肾组织抗氧化功能及元素含量的影响  

Effects of TiO_2 nanoparticles on antioxidant function and element content of liver and kidney tissues in young and adult rats

作　　者：王云[1] 陈章健[1] 巴特[1] 濮吉[1] 崔枭醒[1] 贾光[1] 

机构地区：[1]北京大学公共卫生学院劳动卫生与环境卫生学系,北京100191

出　　处：《北京大学学报（医学版）》2014年第3期395-399,共5页Journal of Peking University:Health Sciences

基　　金：国家重点基础研究发展计划(973计划;2011CB933402);北京市自然科学基金(7132117);高等学校博士学科点专项科研基金(20110001120027)资助~~

摘　　要：目的:比较研究纳米二氧化钛灌胃染毒对幼年和成年大鼠肝、肾组织抗氧化性能及组织中元素含量的影响。方法:将24只4周龄(幼年)和24只9周龄(成年)清洁级雄性SD大鼠按体重用随机数字表法分为8组(每组6只),每天1次灌胃,分别给予0、10、50、200 mg/kg纳米二氧化钛[(75±15)nm,锐钛矿],染毒30 d后取肝、肾组织进行抗氧化性能指标和元素含量的检测。结果:200 mg/kg纳米二氧化钛染毒导致幼年大鼠的肝组织中总超氧化物歧化酶(total superoxide dismutase,T-SOD)活力和肾组织中还原型谷胱甘肽(reduced glutathione,GSH)/氧化型谷胱甘肽(oxidized glutathione,GSSG)比率明显升高,肝组织中Mo、Co、Mn、P元素含量和肾组织中Rb、Na元素含量明显降低。200 mg/kg纳米二氧化钛染毒导致成年大鼠的肝组织中GSH/GSSG比率和Rb元素含量明显升高,Na元素含量明显降低。成年大鼠肾组织中各指标无明显变化。结论:短期经口摄入纳米二氧化钛,可导致大鼠肝、肾组织的抗氧化能力升高和部分元素含量降低,肝组织反应更敏感,幼年大鼠比成年大鼠更易感。Objective：To compare the effect of TiO 2 nanoparticles on antioxidant function and element content of liver and kidney tissues in young and adult rats .Methods：Forty-eight SD male rats , half in 4-week （youth） old and half in 9-week （adult） old rats, were randomly divided into 8 groups, which were exposed to TiO2 nanoparticles [（75 ±15） nm, anatase] through intragastric administration at 0, 10, 50 and 200 mg/kg body weight every day for 30 days.The liver and kidney tissues were collected for antioxidant function and element content analysis .Results： 200 mg/kg TiO2 nanoparticles exposure significantly increased the liver total superoxide dismutase （ T-SOD ） activity and the kidney reduced glutathione （GSH）/oxidized glutathione （GSSG） ratios in young rats, and significantly decreased the liver Mo, Co, Mn and P contents and the kidney Rb and Na contents in young rats .200 mg/kg TiO2 nanoparticles exposure significantly increased GSH/GSSG ratios and Rb contents and decreased Na con-tents in the liver of adult rats .No significantly difference was found in antioxidant indexes and elements content in the kidney of adult rats between three experimental groups and control group .Conclusion：TiO2 nanoparticles can enhance the antioxidant capacity and decrease the elements content in rat liver and kidney tissues .The liver is the more sensitive target organ and the young animals are more susceptible to TiO2 nanoparticles toxicity by the oral routes .

关 键 词：纳米粒子 二氧化钛 安全 肝毒性 肾毒性 

分 类 号：R155.51[医药卫生—营养与食品卫生学]