1-甲基色氨酸与5-氟尿嘧啶联合干预对荷胃癌小鼠髓源性抑制细胞的影响  

Effects of 5-fluorouracil with 1-methyl tryptophan on myeloid-derived suppressor cells in gastric cancer bearing 615 mice

作　　者：王改[1] 申惠琴[2] 武希润[2] 刘小丽[2] 康贵云[2] 李哲萍[2] 王琦[2] 

机构地区：[1]山西医科大学研究生院,太原030001 [2]山西医科大学第二医院消化内科

出　　处：《中华普通外科杂志》2014年第6期464-466,共3页Chinese Journal of General Surgery

摘　　要：目的 研究1-甲基色氨酸（1-methyl tryptophan,1-MT）和5-氟尿嘧啶（5-fluorouracil,5-FU）对荷胃癌小鼠髓源性抑制细胞（myeloid-derived suppressor cells,MDSC）的影响.方法 将稳定转染IDO-pcDNA3.1（＋）质粒和空质粒以及未转染的小鼠前胃癌细胞种植于小鼠皮下,建立荷胃癌移植瘤小鼠模型,分别给予生理盐水、1-MT、5-FU和1-MT＋ 5-FU治疗.取小鼠脾脏及肿瘤组织,用流式细胞术和免疫荧光法检测组织中MDSC的数量.结果 （1）移植瘤中有MDSC的表达.（2）胃癌小鼠体内MDSC（49.8％±1.1％）较正常小鼠（1.2％±0.3％）明显增加（P＜0.05）.（3）与正常小鼠相比,联合用药组MDSC（18.5％±0.5％）的数量明显减少,差异有统计学意义（P＜0.05）.结论 1-MT与5-FU联合应用使荷胃癌移植瘤小鼠脾脏及肿瘤部位的MDSC数量减少,改善肿瘤微环境.Objective To investigate the effect of 1-MT in combination with 5-FU on the number of myeloid-derived supressor cells （MDSC） in gastric carcinoma beating mice.Methods By using the lipofectamine TM 2000,the eukaryotic expression plasmid pcDNA3.1-IDO and empty vector pcDNA3.1 （＋） were transfected in a MFC cell line.Animal model of gastric cancer bearing 615 mice were established to give respectively normal saline （NS）,1-MT,5-FU and 1-MT ＋ 5-FU therapy.Flow cytometry and immunofluorescence were used to analyze MDSC expression in tumor tissue.Result （1） The expression of MDSC was detected in transplanted tumor.（2）The expression of MDSC in gastric cancer beating 615 mice （49.8％ ± 1.1％） was higher than the normal group （1.2％ ± 0.3％） （P ＜ 0.05）.（3） Compared with normal group the MDSC expression in 1-MT ＋ 5-FU group （18.5 ％ ± 0.5 ％） decreased significantly （P ＜ 0.05）.Conclusions Combining 1-MT with 5-FU can reduce the number of MDSC in gastric cancer bearing 615 mice,improving tumor microenvironment.

关 键 词：胃肿瘤 色氨酸 氟尿嘧啶 髓源性抑制细胞 

分 类 号：R735.2[医药卫生—肿瘤]