H_2S与Ca^(2+)协同增强谷子对Cr^(6+)胁迫的耐受  

Ca^(2+) Participates in H_2S Induced Cr^(6+) Tolerance in Setaria italica

作　　者：方慧慧[1] 裴雁曦[1] 田保华[1] 张丽萍[1] 乔增杰[1] 刘志强[1] 

机构地区：[1]山西大学生命科学学院,太原030006

出　　处：《中国细胞生物学学报》2014年第6期758-765,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31372085;31300236);山西省高等学校科技创新基金(批准号:2013103)资助的课题~~

摘　　要：继一氧化氮(NO)和一氧化碳(CO)之后,第三种气体信号分子硫化氢(H2S)对植物体生长发育和环境胁迫应答的调控正在受到越来越多的关注。钙离子(Ca2+)是重要的第二信使,参与植物对多种胁迫的响应。该实验以谷子这种抗逆性较强的作物为材料,对其响应六价铬(Cr6+)胁迫过程中H2S和Ca2+信号的互作进行了研究。结果表明,Cr6+胁迫显著激活谷子幼苗的H2S产生系统,外源H2S预处理能明显降低Cr6+胁迫对谷子根尖细胞的损伤,而H2S的合成抑制剂羟胺(HA)预处理,使得Cr6+对谷子的毒害增强;进一步实验发现,H2S能激活Ca2+信号下游相关基因的表达,同时Ca2+能增强H2S的产生,表明在植物体内H2S和Ca2+信号存在复杂的联系。该研究也证明,H2S和Ca2+可以通过调节重金属离子转运蛋白增强谷子对Cr6+的耐受。Hydrogen sulfide （H2S）, as the third gasotransmitter after nitric oxide （NO） and carbon monoxide （CO）, is attracting more and more attentions by the reason of regulating the growth and development process and stress responses in plants. Calcium （Ca2＋） has been regarded as a core transducer and regulator in many adaptation processes of plants. In this study, the foxtail millet （Setaria italica）, which has considerable tolerance to various stresses, was employed to explore the interaction between HES and Ca2＋ signaling in responding to Cr6＋ stress. The results showed that Cr6＋ stress significantly activated the endogenous HaS generation system. Correspondingly, cell damage from Cr6＋ stress was alleviated by exogenous H2S pretreatment but further aggravated by HES synthesis inhibitor hydroxylamine （HA）. Meanwhile, under normal conditions, exogenous H/S regulated the expression of Ca2＋ signaling downstream molecules coding genes, while the emission of endogenous H2S was also heightened by Ca2＋. Further results demonstrated that H2S and Ca2＋ improved the Cr6＋ tolerance by adjusting the heavy metal ion transporters.

关 键 词：硫化氢 钙信号 铬胁迫 谷子 

分 类 号：X173[环境科学与工程—环境科学] S515[农业科学—作物学]