MicroRNA-200a在肝癌组织中的表达及临床意义  

The expression of microRNA-200a and its clinical significance in hepatic cancer tissues

作　　者：杨西胜 汪建林[1] 李小磊[1] 阮柏[1] 马奔[1] 高远[1] 郭欣[1] 王璟[1] 段涛[1] 李海民[1] 

机构地区：[1]西安第四军医大学西京医院肝胆外科,710032

出　　处：《中华肝胆外科杂志》2014年第6期406-409,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：国家自然科学基金（81172061）;陕西省自然科学基金（2011K3-03-04）

摘　　要：目的探讨microRNA-200a（miR-200a）在肝癌组织中的表达，分析其与临床病理参数及肝癌预后的关系。方法采用实时PCR检测肝癌细胞系和58例肝癌标本及其癌旁组织中miR-200a的表达水平，分析miR-200a表达与临床病理和肝癌患者预后的关系。结果miR-200a在四种肝癌细胞系中表达均低于正常肝脏细胞系，在肝癌组织中表达水平（0．593±0．107）比癌旁组织（1．652±0．135）显著降低（P〈0．05）；miR-200a的表达与肝癌的侵袭转移有关。有肝癌侵袭转移患者组明显低于无侵袭转移组（P=0．026）。而miR-200a的表达与性别、年龄、肝炎病史、肿瘤大小、TNM分期及AFP值无关（均P〉0．05）。低表达miR-200a的肝癌患者预后较差。结论miR-200a在肝癌组织中呈低表达，并与肝癌的侵袭转移和不良预后有关。Objective To determine the expression of microRNA-200a （miR-200a） in hepatic cancer tissues and its clinical significance. Methods The levels of expression of miR-200a in hepatic cancer cell lines, and in 58 cases of hepatic cancer and their adjacent normal liver tissues were detected by real time reverse transcription polymerase chain reaction（RT-PCR）. The relations of the levels of miR-200a expression with clinicopathologic features were analyzed. Results RT-PCR revealed that the expression of miR-200a was down regulated in hepatic cancer cell lines and hepatic cancer tissues. The expression of miR-200a was associated with tumor invasiveness and metastasis in patients with hepatic cancer. The expression of miR-200a in patients with tumor invasiveness and metastasis was significantly lower than those without （P 〈 0.05）. There was no significant difference with regard to sex, age, hepatitis status, tumor size, TNM staging and the level of AFP （all P 〉0.05）. Conclusion The expression of miR-200a was downregnlated in hepatic cancer tissue. There was a significant relation to tumor invasiveness and metastasis.

关 键 词：肝癌组织 核糖核酸 microRNA-200a 侵袭转移 

分 类 号：R735.7[医药卫生—肿瘤]