原发性肝癌患者甲胎蛋白基因多态性与其在外周血中表达水平的相关性  

Relationship between α-fetoprotein polymorphisms and peripheral α-fetoprotein level in hepatocellular carcinoma patients

作　　者：吉强[1] 顾星[1] 周军[1] 殷月鹏[1] 陈冬梅[1] 陈思佳[1] 董郭平 高春芳[1] 

机构地区：[1]第二军医大学东方肝胆外科医院实验诊断科,上海200438

出　　处：《第二军医大学学报》2014年第6期603-609,共7页Academic Journal of Second Military Medical University

基　　金：国家科技重大专项项目(2012ZX10002-016)~~

摘　　要：目的探讨原发性肝细胞癌(hepatocellular carcinoma,HCC)患者甲胎蛋白(α-fetoprotein,AFP)基因多态性与外周血AFP水平的相关性。方法采用聚合酶链反应和连接酶检测反应法(PCR-LDR)检测我院收治的335例HCC患者AFP基因多个位点多态性并检测AFP血清水平,运用SPSS 13.0软件对结果进行统计学分析。结果 AFP基因-205C>G位点GG基因型分布在AFP水平≥20和<20ng/mL两组间差异有统计学意义(GG vs CC:χ2=4.389,P=0.036),G等位基因分布在AFP水平≥400和<400ng/mL两组间差异有统计学意义(G vs C:χ2=4.410,P=0.036)。AFP基因intron1A>T、intron7A>G、3′UTR A>G和intron2A>G基因多态性与AFP基因表达水平无密切相关性。结论在中国汉族人群HCC患者中,AFP基因-205C>G位点C等位基因及CC基因型携带者外周血AFP水平明显升高。Objective To study the relationship between α-fetoprotein （AFP） polymorphisms and peripheral AFP level in hepatocellular carcinoma （HCC） patients. Methods Using polymerase chain reaction-ligation detection reaction （PCR-LDR） technique, we determined the polymorphisms of AFP of 335 HCC patients; the serum levels of AFP were also determined. SPSS 13.0 software was used for statistical analysis. Results The GG genotype frequency of --205 C〉G of AFP gene was significantly different between patients with AFP values 〉20 and %20 ng/mL （GG vs CC： Х^2 = 4. 389, P=0. 036）. The frequency of G allele was also found significantly different between patients with AFP values ≥400 and %400 ng/mL （G vs C： Х^2 =4. 410, P= 0. 036）. No relationship was found between AFP expression and other polymorphisms, including intronl A〉T, intron7 A＆G, 3＇UTR A〉G and intron2 A〉G, in AFP gene. Conclusion In HCC patients among Chinese Han population, the C allele and CC genotype of -205 C〉G polymorphism site in AFP gene is associated with higher AFP values.

关 键 词：甲胎蛋白类 单核苷酸多态性 肝细胞癌 乙型肝炎 

分 类 号：R735.7[医药卫生—肿瘤]