马钱子碱通过阻滞细胞周期抑制人肺癌细胞株PC-9增殖  

Brucine Inhibits the Proliferation of Human Lung Cancer Cell Line PC-9 via Arresting Cell Cycle

作　　者：李苗 李平 张梅 马峰 苏丽 

机构地区：[1]安徽医科大学附属省立医院中医肿瘤科,合肥230001

出　　处：《中国肺癌杂志》2014年第6期444-450,共7页Chinese Journal of Lung Cancer

基　　金：国家中医药管理局中医肿瘤病学重点学科[国中医药发(2009)30号]经费资助~~

摘　　要：背景与目的已有的研究表明:Cyclin D1和Cyclin E是细胞周期中重要的正性调控因子,其高表达与肿瘤的增殖密切相关。本研究旨在探讨马钱子碱(Brucine)对人肺癌细胞株PC-9增殖的影响,及其与Cyclin D1和Cyclin E表达的影响。方法将PC-9细胞分为4组:空白对照组、DMSO对照组(2‰)、150μM Brucine组、300μM Brucine组。CellTiter-Glo发光法、平板克隆形成实验观察该药对PC-9细胞增殖的影响,流式细胞仪检测细胞周期,qRT-PCR检测细胞周期相关基因Cyclin D1、Cyclin E mRNA的表达,Western blot检测细胞周期相关基因Cyclin D1、Cyclin E蛋白的表达。结果与对照组比较,CellTiter-Glo发光法、平板克隆形成实验结果显示:Brucine可以抑制人肺癌细胞株PC-9的增殖,并呈时间-剂量依赖性(P<0.01);流式结果显示对细胞周期的影响主要是阻滞PC-9细胞于G0/G1期;qRT-PCR结果显示Cyclin D1、Cyclin E mRNA的表达下调;Western blot结果显示Brucine使Cyclin D1、Cyclin E的表达降低。结论 Brucine能明显抑制人肺癌细胞株PC-9的增殖,机制主要与其通过下调Cyclin D1、Cyclin E表达,进而阻滞细胞周期有关。Background and objective It has been proven that Cyclin D1 and Cyclin E are the important positive regulators of cell cycle, they are closely related to the tumor proliferation. hTe aim of this study is to explore the relationship between Brucine and the proliferation in human lung cancer cell line PC-9, and the effect of it on the expression of Cyclin D1 and Cyclin E. Methods PC-9 cells were divided to 4 groups：the normal control group, the DMSO control group （2‰）, the 150μM Brucine group, and the 300μM Brucine group. hTe proliferation rate of PC-9 cells was determined by hTe CellTiter-Glo Luminescent Cell Viability Assay and Colony Formation assay. hTe change of cell cycle was detected by Flow cytome try. Expressions of cell cycle regulators Cyclin D1, Cyclin E mRNA were determined by qRT-PCR. Protein expression of cell cycle regulators Cyclin D1, Cyclin E were determined by Western blot. Results Compared with the control, Brucine remarkably in-hibited the proliferation of PC-9 cells in a dose-and time-dependent manner （P〈0.01）;Flow cytome try showed that Brucine blocked the cell cycle of PC-9 cells at G0/G1, and the differences were statistically signiifcant （P〈0.01）;qRT-PCR showed that the expression of Cyclin D1, Cyclin E mRNA were down-regulated;Western blot showed that the protein expression of Cyclin D1, Cyclin E were down-regulated. Conclusion Brucine can inhibit the proliferation of human lung cancer cell line PC-9 mainly by blocking the cell cycle at G0/G1 via down-regulating the expression of Cyclin D1, Cyclin E.

关 键 词：马钱子碱 细胞周期 人肺癌细胞株PC-9 增殖 

分 类 号：R734.2[医药卫生—肿瘤]