阿司匹林对SD大鼠小肠内源性保护因素影响的初探  

Preliminary study on effect of aspirin against endogenous protective factors of intestinal mucosa in SD rats

作　　者：郑桐[1] 杨天飞 车筑平[1] 谭庆华[3] 

机构地区：[1]贵阳医学院附属医院,贵阳550004 [2]贵州省贵阳市贵航贵阳医院,贵阳550006 [3]四川大学华西医院,成都610000

出　　处：《中国免疫学杂志》2014年第6期726-730,共5页Chinese Journal of Immunology

基　　金：贵州省科技厅基金(E2011-10)

摘　　要：目的:探讨非甾体类消炎药(Non-steroid anti-inflammatory drugs,NSAIDs)阿司匹林注射液对SD大鼠回肠生长抑素(Somatostatin,SST)、表皮生长因子受体(Epidermal growth factor receptor,EGFR)、前列腺素E2(Prostaglandin E2,PGE2)及黏液影响。方法:取32只SD大鼠随机分为灌胃组、灌肠组、腹腔注射组及空白对照组四组,每组8只。对回肠黏膜损伤进行评分,阿利辛蓝染色显示其黏液的分布并测定阳性面积及积分光密度(Integrated option density,IOD)值;ELISA检测各组回肠组织SST、EGFR、PGE2水平;免疫组化检测各组回肠组织SST、EGFR、PGE2的分布,图像分析软件测定IOD值。结果:阿司匹林治疗组肠黏膜损伤指数比正常对照组高(P<0.05),两两比较黏膜损伤评分无差别;其黏液的面积和IOD值均比正常对照组低(P<0.05),其中腹腔注射组的面积(602.17±158.03)及IOD值(249.54±113.19)最低(P<0.05)。四组间的SST、EGFR浓度、IOD值差异无统计学意义,四组间PGE2浓度、IOD值相比较,空白对照组最高(P<0.05),三组阿司匹林组间两两比较其水平没有差异。结论:阿司匹林在不同给药途径下,2周均可引起大鼠小肠黏膜的损伤。这种损伤中,小肠黏膜的完整性破坏和黏膜黏液的分泌受到了明显影响,而腹腔注射给药对小肠黏膜黏液的合成和分泌影响最大,可能这些改变与PGE2的降低有关。无明确结果表明SST和EGFR参与了NSAIDs性肠病的病理生理过程。Objective：To investigate the changes of somatostatin ,epidermal growth factor receptor ,prostaglandin E2 and mucus of ileum in SD rats injected aspirin.Methods：32 SD rats were randomly divided into 4 groups：oral intake group ,coloclyster group ,in-traperitoneal injection group and normal control group.Ileal tissue sections were stained by Alcian blue.The positive area and IOD of Alcian blue was counted by image analysis in each group.The concentration of SST ,EGFR and PGE2 were detected by ELISA immuno-histochemistry ,and the IOD of each index was measured by image analysis in each group.Results：The score of ileum mucosal injury were highest in aspirin treatment groups （ P〈0.05 ） ,but the score was no difference among aspirin treatment groups.The area and IOD of mucus in aspirin treatment groups were less than normal control group .The Area （ 602.17 ±158.03 ） and level of IOD （ 249.54 ± 113.19） in intraperitoneal injection group was the lowest （P〈0.05）.There were no differences of level of SST ,EGFR was observed among all groups .The concentration and IOD of PGE2 in normal control group was the highest （P〈0.05）.However,his discrepancy was not obvious among three groups of aspirin.Conclusion： Aspirin, in the four routes of administration , can cause damage to the mucosa of the rat small intestine in two weeks.The integrity of the small intestinal mucosa and mucus secretion were damaged significantly ,and it was the worst in intraperitoneal injection group.These changes might be correlated with the reduction of PGE 2.No clear results showed that the SST and EGFR were involved in the pathophysiology of the NSAIDs .

关 键 词：阿司匹林 肠黏膜屏障 NSAIDs性肠病 前列腺素E2 生长抑素 表皮生长因子受体 

分 类 号：R574.53[医药卫生—消化系统]