滋养细胞疾病中CyclinE及其相关蛋白的表达  

Expression of Cyclin E and Its Related Proteins in Gestational Trophoblastic Disease

作　　者：李娜[1] 曲芃芃[1] 

机构地区：[1]天津市中心妇产科医院,300100

出　　处：《国际妇产科学杂志》2014年第3期324-326,共3页Journal of International Obstetrics and Gynecology

摘　　要：目的:探讨细胞周期蛋白E(Cyclin E)、细胞周期蛋白依赖激酶2(CDK-2)、P27KIP1和P21WAF1/CIP1在正常胎盘组织、葡萄胎和滋养细胞肿瘤(gestational trophoblastic neoplasia,GTN)组织中的表达,研究上述指标表达水平的相关性以及与妊娠滋养细胞疾病(gestational trophoblastic disease,GTD)的关系。方法:采用链霉素-过氧化物酶免疫组化方法检测30例正常胎盘组织、38例葡萄胎组织和9例GTN组织(侵蚀性葡萄胎8例,绒癌1例)中Cyclin E、CDK-2、P27KIP1和P21WAF1/CIP1的表达,分析其与GTD发生发展及预后的关系。结果:①Cyclin E、CDK-2在葡萄胎和GTN组织中的表达高于正常胎盘组织(P<0.01);P27KIP1和P21WAF1/CIP1在GTN组织中的表达低于葡萄胎和正常胎盘组织(P<0.05),P27KIP1和P21WAF1/CIP1在发生恶性转变的葡萄胎组织中的表达低于未发生恶变者(P<0.05)。②Cyclin E与P27KIP1、P21WAF1/CIP1的表达呈负相关(P<0.05);Cyclin E与CDK-2的表达呈正相关(P<0.05);P27KIP1与P21WAF1/CIP1的表达呈正相关(P<0.05)。CDK-2表达随P27KIP1和P21WAF1/CIP1的表达降低呈增高趋势,但差异无统计学意义(P>0.05)。结论:Cyclin E/CDK-2复合物及其抑制蛋白P27KIP1和P21WAF1/CIP1的异常表达参与了GTN的发生发展;P27KIP1和P21WAF1/CIP1可能成为预测葡萄胎恶变的有效标记物。Objective： To investigate the expression of Cyclin E,CDK-2 and their inhibitors P27KIP1 and P21WAF1/CIP1 in gestational trophoblastic disease （GTD）. Methods：Expression of the above indexes were determined immunohistochemically by specific antibodies for these proteins on formalin-fixed paraffin sections of 30 of normal placenta tissues ,38 of hydatidiform moles and 9 of gestational trophoblastic neoplasms （GTN）, including 8 invasive moles and 1 choriocarcinoma. Results：①The expression of Cyclin E and CDK-2 in GTD was significantly higher than that in normal placentas （P〈0.01）. The expression of P27KIP1 and P21WAF1/CIP1 in GTN was significantly lower than that in hydatidiform moles and normal placenta tissues ,respectively （P〈0.05）. Moreover,the expression of P27KIP1 and P21WAF1/CIP1 in hydatidiform moles which developed GTN was significantly lower than those spontaneously regressed. ②Significant negative correlations between the Cyclin E and P27KIP1,P21WAF1/CIP1 staining indexes were observed （P〈0.05）. There was a positive correlation between the expression of CDK-2 and Cyclin E （P〈0.05）. Also,the expression of P27KIP1 and P21WAF1/CIP1 showed positive correlation （P〈0.05）. CDK-2 showed inverse expression pattern compared with P27KIP1 and P21WAF1/CIP1,but no statistical difference was observed （P〉0.05）. Conclusions：Disregulation of Cyclin E/CDK-2 complex and its inhibitors participated in the development of gestational trophoblastic tumor. P27KIP1 and P21WAF1/CIP1 may be used as effective indicators for the prognosis of hydatidiform moles.

关 键 词：妊娠滋养细胞肿瘤 细胞周期蛋白依赖激酶2 P27KIP1 P21WAF1 CIP1 

分 类 号：R737.33[医药卫生—肿瘤]