非洲紫罗兰F3′5′H基因的克隆及烟草的转化分析  

Cloning of flavonoid-3′,5′-hydroxylase gene in Saintpaulia ionantha and expression analysis of transgenic Nicotiana tabacum

作　　者：焦阳[1] 陈洁[1] 贾海燕[1] 张克[1] 杨凯[1] 王文和[1] 

机构地区：[1]北京农学院园林学院,北京102206

出　　处：《北京农学院学报》2014年第3期11-13,共3页Journal of Beijing University of Agriculture

基　　金：北京市园林绿化局计划项目(YLHH201400102);北京市教委项目(PXM2013-014207-00079)

摘　　要：本研究利用PCR技术克隆了非洲紫罗兰（Saintpaulia ionantha）花色合成的关键酶,F3＇5＇H的基因.对克隆序列的分析表明,F3＇5＇H基因序列上非洲紫罗兰与金鱼草（Antirrhinum Kelloggii）、矮牵牛（ToreniaH ybrida）有着较近的亲缘关系.构建了该基因的pCAMBIA1304表达载体,采用农杆菌介导叶盘法转化普通烟草（Nicotiana tabacum）,获得了转基因植株.利用高效液相色谱法（HPLC）法检测转基因植株类黄酮含量,转基因烟草类黄酮相对含量升高4.0％～16.3％,同时花色向紫色转变.表明该基因对植物花色的形成有重要作用,可为基因工程蓝色花育种提供理论依据和基础.In the research the Flavonoid-3＇ ,5＇-hydroxylase gene （F3＇5＇ H） was isolated from Saintpaulia by PCR method. Se- quence analysis showed that Saintpaulia ionantha, Antirrhinum Kelloggiiand Torenia Hybrida have similar relationship, and the sense F3＇5＇Hexpression vectors pCAMBIA 1304 was constructed and transformed into tobacco by Agrobacterium media- ted. In tansgenic tobacco, the chaocone content was detected by High Performance Liquid Chromatograaphy （HPLC）, the relacrive content of chaocone were 4.0%-16.3% higher then that of wildtype, and the flower colour was changed to heliotrope. The results indicated that F3P5＇H play a key role of flower colour regulation, and provide a theoretical reference for blue flower molecular breeding.

关 键 词：类黄酮3’5’羟基化酶(F3’5’H) 载体构建 烟草转化 类黄酮 

分 类 号：S682.2[农业科学—观赏园艺]