特异性siRNA抑制XIAP基因表达对人内膜癌细胞凋亡的影响  

Effect of Specific siRNA Inhibition of XIAP Expression on Cell Apoptosis of Human Endometri-al Carcinoma

作　　者：银铎[1] 王宁[1] 张淑兰[1] 鲁艳明[1] 李威[1] 霍乃晨[1] 肖倩[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004

出　　处：《医学临床研究》2014年第5期833-836,共4页Journal of Clinical Research

基　　金：国家自然科学基金（No.81202047）;辽宁省自然科学基金计划（No.201202265）

摘　　要：【目的】探讨RNA干扰抑制内膜癌RL95-2细胞XIAP基因表达及对细胞凋亡的影响。【方法】设计并合成XIAP基因特异性siRNA，转染人内膜癌RL95-2细胞，Real time RT-PCR检测转染后XIAP mRNA的变化，Western Blot检测 XIAP蛋白的变化，MTT 及流式细胞仪法检测细胞增值和凋亡的变化。【结果】XIAP siRNA转染后，特异性转染组mRNA转录量的相对倍数值为0．04±0．06，相对蛋白表达量分别为0．590±0．178，较各对照组明显减少（P＜0．05）；特异性转染组细胞生长抑制率为（47．86±4．46）％，较对照组明显增高（P＜0．05）。【结论】体外实验表明，合成的 siRNA能在mRNA水平及蛋白水平有效抑制人内膜癌RL95-2细胞内 XIAP基因的转录及表达，进而显著的促进内膜癌细胞凋亡，其促凋亡机制仍有待进一步研究。[Obj ective]To explore the effect of RNA interference（RNAi）targeting XIAP gene on cell ap-optosis of human endometrial carcinoma.[Methods]Specific small interference RNA（siRNA）of XIAP was de-signed and composed.Endometrial cancer cell line RL95-2 was transfected.The changes of mRNA and protein of XIAP after transfection were detected by real time RT-PCR and Western Blotting,respectively.MTT and flow cytometry method were used to detect cell proliferation and apoptosis.[Results]After transfection of XI-AP siRNA,the relative fold value of mRNA transcription volume and the relative protein expression volume in specific transfection group were （0.04±0.06）and （0.590±0.178）respectively,which were obviously lower than those in the control group（P〈0.05）.The cell growth inhibition rate in specific transduction group was （47.86±4.46）%,which was obviously higher than that in control group（P〈0.05）.[Conclusion]The ex-periment in vitro indicates that the composed siRNA can effectively inhibit the transcription and expression of XIAP in human endometrial cancer cell line RL95-2,and further promote cell apoptosis of endometrial carcino-ma significantly.The mechanism of promoting cell apoptosis still needs further investigation.

关 键 词：RNA 小分子干扰 基因表达 细胞凋亡 

分 类 号：R730[医药卫生—肿瘤]