壳梭孢素对铝敏感型黑大豆铝耐受性的影响  

Effects of Fusicoccin on Aluminum Tolerance in Al-sensitive Black Soybean

作　　者：易嘉[1] 郭传龙[1] 武孔焕[1] 王琳[1] 赵秀玲[1] 李昆志[1] 陈丽梅[1] 

机构地区：[1]昆明理工大学生物工程技术研究中心,云南昆明650500

出　　处：《大豆科学》2014年第3期389-392,共4页Soybean Science

基　　金：国家重点基础研究发展计划"973计划"(2007CB108901)

摘　　要：以铝敏感型黑大豆(简称SB)为材料,研究铝胁迫下壳梭孢素(FC)对SB根铝耐受性的影响。结果表明:在50μmol·L-1铝胁迫下,添加1μmol·L-1FC处理8 h能够显著缓解铝胁迫对SB根生长的抑制作用,使其根的相对生长率(RRG)增加36.4%。免疫共沉淀分析结果表明50μmol·L-1铝胁迫下添加1μmol·L-1FC增强SB根中14-3-3蛋白与磷酸化质膜H+-ATPase的结合,使根尖质膜H+-ATPase活性提高约1倍,根氢泵活性和质子外排氢离子能力显著增强,根尖柠檬酸的分泌量增加显著,增加约2倍。这些结果证实铝胁迫下FC用于增强SB根中14-3-3蛋白和质膜H+-ATPase的相互作用和质膜H+-ATPase的活性,从而增加SB根柠檬酸的分泌作用及其对铝胁迫的耐受性。Effect of FC on aluminum tolerance of Al-sensitive black soybean （ SB ） roots under Al stress were investigated in this research. The results showed that adding 1 μmol·L^-1 FC for 8 h, it could significantly release the inhibition of 50 μmol·L^-1 Al stress on the root growth of SB, and increase its relative root growth（RRG） by 36.4%. Co-Immunoprecipitation （Co-IP） analysis showed that adding 1 μmol·L^-1 FC could enhance the interaction between 14-3-3 proteins and phosphorylated PM H ^＋ -ATPase, which increased PM H^＋ -ATPase activity to approximately 1 -fold, significantly increasing proton efflux and citrate secretion to approximately 2-fold in SB roots under 50 μmol·L^-1 Al stress. These results confirmed FC was used for enhancing interaction of the 14-3-3 protein and PM H ^＋ -ATPase and the activity of PM H^＋-ATPase, thereby increasing citrate secretion and Al-tolerance in SB roots under Al stress.

关 键 词：黑大豆 14-3-3蛋白 质膜H^+-ATPASE 壳梭孢素 铝胁迫 

分 类 号：S565.1[农业科学—作物学]