MMP-2和MMP-9在急性B淋巴细胞白血病中的表达及临床意义  

Expression and Clinical Significance of MMP-2 and MMP-9 in B Acute Lymphoblastic Leukemia

作　　者：潘玉夏[1] 杨琳[1] 温树鹏[1] 刘小军[1] 尚银涛[1] 王兴哲[1] 王茜[1] 杨敬慈[1] 罗建民[1] 

机构地区：[1]河北省血液病重点实验室,河北医科大学第二医院血液内科,河北石家庄050000

出　　处：《中国实验血液学杂志》2014年第3期640-643,共4页Journal of Experimental Hematology

摘　　要：本研究探讨急性B淋巴细胞白血病(B-ALL)患者骨髓细胞中基质金属蛋白酶-2和-9(MMP-2,MMP-9)的表达及临床意义。采用半定量RT-PCR方法检测初治、复发B-ALL患者和正常对照骨髓中单个核细胞MMP-2,MMP-9 mRNA的表达。采用明胶酶谱检测B-ALL患者和正常对照骨髓单个核细胞孵育上清中MMP-2,MMP-9的酶活性。结果表明,初治、复发组B-ALL患者中MMP-2表达率明显高于正常对照组(P<0.05);MMP-9表达率明显低于正常对照组(P<0.05)。髓外浸润组患者MMP-2和MMP-9表达率均明显高于无髓外浸润组(P<0.05),而MMP-2/MMP-9(+)组髓外浸润发生率明显高于MMP-2/MMP-9(-)组。高危组B-ALL患者MMP-9表达率明显高于标危组,而两组间MMP-2表达率无显著性差异(P>0.05)。结论:B-ALL患者白血病细胞可以产生MMP-2,MMP-9,可能参与了B-ALL的髓外浸润,MMP-9表达可能提示预后不良。This study was purposed to investigate the expression and clinical significance of MMP-2 and MMP-9 in patients with B-acute lymphoblastic leukemia （B-ALL）. The expression of MMP-2 and MMP-9 in bone marrow mononuclear cells of B-ALL patients and normal controls was detected by RT-PCR. The gelatinolytic activity was detected by zymography. The results showed that the expression of MMP-2 in de novo and relapsed B-ALL patients was markedly higher than that in normal controls （P 〈0.05）. The expression of MMP-9 in de novo and relapsed B-ALL patients was markedly lower than that in normal controls （ P 〈 0.05 ）. The expression of MMP-2 and MMP-9 in patients with extramedullary infiltration was significantly higher than that in patients without extramedullary infiltration. The incidence of extramedullary infiltration in patients with MMP-2/MMP-9 （ ＋ ） was markedly higher than that in patients with MMP-2/ MMP-9 （ - ）. The expression of MMP-9 was markedly higher in high-risk patients than that in standard-risk patients （ P 〈 0.05 ）, but the expression of MMP-2 had no significant difference between the high-risk and standard-risk patients （P 〉 0.05 ）. It is concluded that MMP-2 and MMP-9 may be secreted by B lymphoblasts and may involve in the extramedullary infiltration. MMP-9 may correlate with ooor prognosis.

关 键 词：B淋巴细胞白血病 MMP-2 MMP-9 

分 类 号：R733.71[医药卫生—肿瘤]