CD37在恶性B细胞中表达意义的探讨  

Significance of CD37 Expression in Malignant B Cells

作　　者：王蔚[1] 李燕[1] 高丽[1] 徐韶华[1] 龚明[1] 黄泛舟[1] 李振玲[1] 陈艳荣[1] 马一盖[1] 

机构地区：[1]卫生部中日友好医院血液科,北京100029

出　　处：《中国实验血液学杂志》2014年第3期644-647,共4页Journal of Experimental Hematology

摘　　要：本研究旨在探讨CD37抗原在急性B淋巴细胞白血病(B-ALL)及B细胞非霍奇金淋巴瘤(B-NHL)细胞中表达的意义。利用多参数流式细胞仪检测对照骨髓标本、B-ALL标本及B-NHL标本细胞中CD37的表达。结果表明,20例B-ALL细胞[初治5例,伴微小残留病(MRD)15例]低表达CD37(1.04±0.24;1.50±0.89),正常前体B细胞也低表达CD37(1.64±0.52),3组间CD37表达水平无统计学差异(P>0.05)。正常成熟B细胞和BNHL细胞均高表达CD37(14.23±7.84和14.53±10.93),它们之间差异无统计学意义(P>0.05)。正常B细胞不同发育阶段的CD37表达水平比较显示,B祖细胞低表达CD37(0.88±0.17),B前体细胞CD37表达增高(2.44±0.69),成熟B细胞CD37表达最高。结论:低表达CD37并非B-ALL细胞所特有。幼稚B细胞低表达CD37,随着B细胞的成熟CD37的表达强度逐渐升高,亦即CD37在B细胞中的表达与细胞的良恶性无关。The aim of this study was to clarify the clinical significance of CD37 expression in B cells from B acute lymphoblastic leukemia （B-ALL） and B cell non-Hodgkin＇s lymphoma （B-NHL）. The expression level of CD37 on B cells from bone marrow samples of normal controls （ n = 19）, B-ALL patients E including untreated cases （ n = 5 ） and cases with minimal residual disease （MRD, n = 15） ] and B-NHL patients （ n = 25 ） whose bone marrow was involved by lymphoma cells, was detected by multiple parameter flow cytometry. The results indicated that the B cells from both untreated cases and cases with MRD lowly expressed CD37 （ 1.04 ± 0. 24 and 1.50 ± 0. 89 ）, the normal precursor B cells （control cases） also lowly expressed CD37 （1.64 ± 0. 52 ）. There was no difference of CD37 expression level between 3 groups of cases（P 〉 0.05）. Meanwhile the normal mature B cells and B-NHL cells highly expressed CD37 （ 14. 23 ± 7. 84 and 14. 53 ±10. 93 ）, but there was no difference of CD37 expression between them （ P 〉 0.05 ）. The comparison of CD37 expression level in normal B cells of development stages showed that the progenitor B cells lowly expressed CD37 （0. 88 ± 0. 17 ）, the CD37 expression of precursor B cells was enhanced （2.44 ± 0. 69 ）, while the CD37 expression level of mature B cells was highest. It is concluded that the low expression of CD37 is not the characteristic of B-ALL cells. The expression level of CD37 increases gradually during the mature process of B cells, i. e, the expression level of CD37 does not assosiate with benignity or malignance of B cells.

关 键 词：CD37 恶性B细胞 急性B淋巴细胞白血病 B细胞非霍奇金淋巴瘤 

分 类 号：R733.71[医药卫生—肿瘤]