5-杂氮-2'-脱氧胞苷对人HL-60细胞DAPK基因表达的影响  

Effect of 5-Aza-2'-deoxycytidine on DAPK Gene Expression in Human HL-60 Cells

作　　者：王春艳[1] 刘文君[2] 

机构地区：[1]遂宁市中心医院儿科,629000 [2]泸州医学院附属医院儿科,四川泸州646000

出　　处：《中国实验血液学杂志》2014年第3期717-722,共6页Journal of Experimental Hematology

基　　金：四川省科技支撑项目(2008SG0035)资助

摘　　要：本研究旨在观察甲基化转移酶抑制剂5-杂氮-2'-脱氧胞苷(5-aza-2'-deoxycytidine,5-aza-2dC)对人急性髓系白血病细胞株HL-60凋亡及死亡相关蛋白激酶(death associated protein kinase,DAPK)基因表达的影响,探讨5-aza-2dC治疗AML的作用机制。不同浓度的5-aza-2dC处理HL-60细胞后,采用瑞式染色法观察5-aza-2dC对HL-60细胞形态的影响,流式细胞术检测5-aza-2dC对HL-60细胞凋亡的影响,RT-PCR法检测5-aza-2dC对HL-60细胞DAPK基因表达的影响。结果表明,①5-aza-2dC呈浓度依赖性地促进HL-60细胞凋亡;②经5-aza-2dC处理后,HL-60细胞DAPK基因表达水平较处理前呈剂量依赖性地增加。结论:随着5-aza-2dC作用浓度的增加,HL-60细胞的凋亡率及DAPK基因表达水平均逐渐增加,提示DAPK基因可能是5-aza-2dC诱导HL-60细胞凋亡的调控基因之一。This study was aimed to investigate the effect of methylation transferase inhibitor 5-aza-2＇-deoxycytidine （5-aza-2dC） of different concentrations on the apoptosis of human acute myeloid leukemia （AML） cell line HL-60 and the expression of DAPK gene in HL-60 cells, as well as to explore the possible anti-AML mechanism of 5-aza-2dC. HL- 60 ceils were treated by 5-aza-2dC of different concentrations. The effect of 5-aza-2dC on the HL-60 cell morphology was observed by Wright＇s staining. The effect of 5-aza-2dC on HL-60 cell apoptosis and DAPK mRNA expression was detected by flow cytometry and reverse transcription-polymerize chain reaction （RT-PCR） respectively. The results showed that the 5-aza-2dC induced the apoptosis of HL-60 cells in a concentration-dependant manner; the 5-aza-2dC increased the expression levels of DAPK mRNA in HL-60 cells in a concentration-dependant manner. It is concluded that the apoptosis rate of HL-60 cells and DAPK mRNA expression level displayed a rising trend with 5-aza-2dC concentration increasing. Therefore, DAPK gene may participate in HL-60 cell apoptosis induced by 5-aza-2dC.

关 键 词：HL-60细胞 5-杂氮-2’-脱氧胞苷 凋亡 DAPK基因 

分 类 号：R733.7[医药卫生—肿瘤]