米诺环素调控P2X7受体抑制BV-2细胞活化的研究  

Minocycline inhibits BV-2 cell activation by regulating P2X7 receptor

作　　者：刘淑琼[1] 杨炼红[1] 蒋龙元[2] 叶晋豪[1] 

机构地区：[1]中山大学孙逸仙纪念医院神经内科,广东广州510120 [2]中山大学孙逸仙纪念医院急诊科,广东广州510120

出　　处：《中国病理生理杂志》2014年第6期1066-1069,共4页Chinese Journal of Pathophysiology

基　　金：中山大学学生科研基金资助项目(No.55);广东省大学生创新实验项目(No.1055811198)

摘　　要：目的：探索P2X7受体在米诺环素（Mino）抑制脂多糖（LPS）刺激的BV-2细胞活化中的作用。方法：将体外培养的BV-2细胞分为5组：空白对照组、LPS处理组、LPS＋0．1μmol／L Mino组、LPS＋1μmol／L Mino组和LPS＋10μmol／L Mino组。分组处理8 h后行real-time PCR检测P2X7受体mRNA表达；处理24 h后观察各组细胞形态学变化，Western blotting检测P2X7受体蛋白表达，取细胞培养液上清行ELISA检测TNF-α和IL-1β的分泌情况。结果：经LPS处理后，BV-2细胞P2X7受体mRNA及蛋白的表达均升高，细胞培养液上清的TNF-α和IL-1β表达升高，同时伴有形态学的改变，而0．1～10μmol／L Mino 能抑制这一趋势，差异有统计学意义（ P ＜0.01）。结论：Mino抑制BV-2细胞活化的机制可能与抑制P2X7受体的活性相关。AIM： To explore the role of P2X7 receptor in inhibition of lipopolysaccharide （LPS）-stimulated BV-2 cell activation by minocycline .METHODS：BV-2 cells were divided into 5 groups：control group, LPS group, LPS＋0.1 μmol/L Mino group, LPS＋1 μmol/L Mino group and LPS＋10 μmol/L Mino group.The expression of P2X7 re-ceptor was determined by real-time PCR and Western blotting .The levels of TNF-αand IL-1βin the microglia culture su-pernatants were measured by ELISA .The morphological changes of the cells were also observed .RESULTS： After ex-posed to LPS, the expression of P2X7 receptor increased in BV-2 cells at mRNA and protein levels .The concentrations of TNF-αand IL-1βin the microglia culture supernatants also increased .Meanwhile, 0.1-10μmol/L minocycline inhibited those changes in a dose-dependent manner .CONCLUSION：Minocycline inhibits the activation of microglia .The mecha-nism may be related to the P2X7 receptor.

关 键 词：米诺环素 受体 P2X7 肿瘤坏死因子α 白细胞介素1Β 

分 类 号：R363[医药卫生—病理学]