CXCR4和CathepsinD在恶性纤维组织细胞瘤中的表达及意义  

Expression of CXCR4 and CathepsinD in malignant fibrous histiocytoma and their biological significance

作　　者：班新超[1,2,3] 孙保存[1,3] 谷彦军[1,3] 魏秀平[1,3] 娄丹[1,3] 李庆选[2] 郑燕君[1] 

机构地区：[1]天津医科大学病理学教研室,天津300070 [2]天津市天津医院病理科,天津300211 [3]天津医科大学肿瘤医院病理科,天津300060

出　　处：《天津医科大学学报》2014年第4期264-266,共3页Journal of Tianjin Medical University

基　　金：国家自然科学基金面上项目(81172046)

摘　　要：目的:探讨CXCR4和组织蛋白酶D(Cathepsin D)的表达与恶性纤维组织细胞瘤(MFH)转移及预后的关系。方法:选取存档的55例MFH组织标本,根据临床资料分为有转移组和无转移组,利用免疫组织化学染色检测两组原发灶中CXCR4和CathepsinD蛋白的表达情况,比较其过表达患者与低表达患者的中位生存期;利用Real-time PCR检测20例MFH有转移组和无转移组原发灶中CXCR4和CathepsinD的mRNA表达水平。结果:CXCR4和CathepsinD表达水平在转移组均显著高于无转移组(P<0.05);CXCR4过表达者的中位生存期均显著低于其低表达者(P<0.05);CathepsinD过表达者的中位生存期低于其低表达者,但差异无显著性(P>0.05)。结论:过表达CXCR4和CathepsinD与MFH的远处转移有关,CXCR4可作为MFH预后不良的参考指标。Objective： To investigate the expression of CXCR4 and CathepsinD in malignant fibrous histiocytoma （MFH） and to explore their relationship with metastasis and prognosis. Methods： Fifty-five MFH cases were divided into two groups （with or without metastasis）. The expression levels of CXCR4 and CathepsinD were detected using immunohistochemical staining method to compare the median survival time of patients with overexpression and underexpression of the two proteins; mRNA expression of CXCR4 and CathepsinD were detected using Real-time RCR in 20 MFH cases with and without metastasis. Results：The protein and mRNA expression levels of CXCR4 and CathepsinD in group with metastasis were significantly higher than those without metastasis （P〈0.05）; the median survival time of patients with overexpression of CXCR4 was significantly less than those with low-expression （P〈0.05）; the median survival time of patients who overexpressed CathepsinD was less than those who underexpressed. However, no statistical significance was found （P〉0.05）. Conclusion：The overexpression of CXCR4 and CathepsinD in MFH is related to distant metastasis and CXCR4 could be associated with poor prognosis of MFH.

关 键 词：恶性纤维组织细胞瘤 转移 CXCR4 CATHEPSIND 

分 类 号：R73[医药卫生—肿瘤]