用于紫菜无性系种质鉴定的计算机化DNA指纹的建立  

Construction of computerized DNA fingerprinting for identification of Porphyra lines

作　　者：贾建航[1] 陈一华[1] 石金锋[1] 金德敏[1] 许璞[2] 梅俊学[3] 翁曼丽[1] 王斌[1] 

机构地区：[1]中国科学院遗传研究所,北京100101 [2]江苏海洋水产研究所,江苏南通226007 [3]中国科学院海洋研究所,山东青岛266071

出　　处：《海洋学报》2001年第1期79-84,共6页

基　　金：国家"863"计划海洋领域资助项目(819-03-02)

摘　　要：研究用 ＲＡＰＤ技术对条斑紫菜（Ｐｏｒｐｈｙｒａ　ｙｅｚｏｅｎｓｉｓ）、坛紫菜（Ｐ． Ｈａｉｔａｎｅｎｓｉｓ）。半叶紫菜（Ｐ．Ｋａｔａｄａｉ ｖａｒ． Ｈｅｍｉｐｈｙｌｌａ）和少精紫菜（Ｐ．Ｏｌｉｇｏｓｐｅｒｍａｔａｎｇｉａ）的１５个无性系丝状体进行了遗传多样分析，用１２０个Ｏｐｅｒｏｎ引物进行了筛选，从中选用来自ＯＰＪ－１８和ＯＰＮ—０２扩增出的８条多态性条带构建了这１５个紫菜无性系的ＤＮＡ指纹图谱，在该图谱中每个紫菜无性系均有各自特异的ＤＮＡ指纹．用１和０分别表示ＤＮＡ带的出现和缺失，将各个无性系的ＤＮＡ指纹用计算机语言表示，建立了１５个紫菜无性系的计算机化ＤＮＡ指纹；在此基础上设计了紫菜无性系种质鉴定的计算机软件ＰｈＧＩ．RAPD (randomly amplified polymorphic DNA) analysis was performed with filaments of 15 Porphyra lines representing P. Yezoensis, P. Haitanensis, P. Katadai var. hemiphylla and P. oligospermatangia. Eight stable and repeatable RAPD bands amplified with two primers, OPN - 02 and OPJ - 18, were selected for the construction of DNA fingerprinting. The RAPD results are scored based on the presence or absence of each of the 8 bands and then converted to computer language expressed with two digitals, 1 and 0, which represent the presence (numbered as l) or absence (numbered as 0) of each band, respectively. Baed on these results, a model DNA fingerprint and a computerized DNA fingerprint are constructed. In the constructed DNA fingerprint, each Porline has its unique fingerprinting pattern and can be easily distinguished from each other. Later, a software, named as PhGI, is designed bed on this DNA fingerprinting for Porphyra line identification.

关 键 词：计算机化 DNA指纹 种质鉴定 RAPD 紫菜 无性系种 植物 分子遗传学 

分 类 号：Q949.292.1[生物学—植物学] Q789