乳浆大戟提取物对人肺癌细胞生长的影响  

Effect of extracts of Euphorbia esula on proliferation of human lung cancer cells

作　　者：王爱红[1] 庞秋霞[1] 陈美霓[1] 米志宽[1] 符兆英[1] 杨文杰[2] 向云涛[2] 

机构地区：[1]延安大学医学院生物化学教研室,延安716000 [2]延安大学医学院

出　　处：《山西医科大学学报》2014年第6期460-464,548,共6页Journal of Shanxi Medical University

基　　金：陕西省教育厅科学研究基金资助项目(12JK0755);延安市科技发展计划资助项目(2010ks-06);国家大学生创新基金资助项目(201210719012)

摘　　要：目的观察乳浆大戟提取物对体外培养的人肺癌细胞A549增殖的影响及其可能的作用机制。方法不同浓度的乳浆大戟提取物(0.2,0.4,0.8 g/L)作用于体外培养的A549细胞,MTT法观察乳浆大戟提取物对A549细胞增殖的影响;Hoechst33528和PI染色法观察细胞凋亡的形态学改变;流式细胞术检测A549细胞的凋亡率变化;JC-1染色法观察细胞线粒体膜电位改变,RT-PCR检测bcl-2、bax的mRNA表达变化。结果乳浆大戟提取物抑制A549细胞的增殖,具有时间和剂量效应关系,0.8 g/L乳浆大戟提取物作用A549细胞48 h后抑制率为46.3%;流式细胞仪结果显示,乳浆大戟提取物作用48h后,可以诱导A549细胞凋亡,凋亡率可达48.4%;荧光显微镜下观察到线粒体膜电位下降;RT-PCR结果显示bcl-2的mRNA表达水平下调,bax的mRNA表达水平上调,其作用呈剂量效应关系。结论乳浆大戟提取物抑制A549细胞增殖是通过线粒体途径诱导A549细胞凋亡,可能的机制是上调bax及下调bcl-2的表达。Objective To investigate the effect of extracts of Euphorbia esula on proliferation of the human lung cancer cell lines A549 cells and explore its related mechanisms. Methods The human lung cancer cell lines A549 cells were treated with different concentrations（0.2,0.4,0.8g/L） of extracts of Euphorbia esula in vitro. The cell proliferation was analyzed by MTr. The cell morphological changes were observed using Hoechst33528 and PI staining. The effect of extracts of Euphorbia esula on apoptosis was observed by flow cytometry. Mitocboudrial membrane potential was observed under fluorescence microscope. RT - PCR methods were used to explore the mRNA expression levels of bcl-2,bax. Results Extracts of Euphorbia esula inhibited the growth of A549 ceils in a time-and dose-dependent manner, and the maximum inhibition rate was 46.3% after treated with 0.8 g/L extracts of Euphorbia esula for 48 h. Extracts of Euphorbia esula induced the apoptosis of A549 cells ,and the apoptotic rate was 48.4% after treated with 0.8 g/L extracts of Euphor- bia esula for 48 h. Fluorescence microscopy showed that mitocbondrial membrane potential decreased. RT-PCR analysis showed that the mRNA expression level of bcl-2 was down-regulated,while the level of bax was up - regulated. Conclusion Extracts of Euphorbia esula can induce the apoptosis of A549 cells by mitocbondrial pathway,which may be related to the increase of bax expression and the decrease of bcl-2 expression.

关 键 词：乳浆大戟提取物 A549细胞 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]