美洲大蠊提取物对肝纤维化大鼠NF-κB和α-SMA表达的影响  

Effect of American cockroach extract on NF-κB andα-SMA expression in CCl4 caused liver fibrosisrat model

作　　者：刘丽辉[1] 李武[1] 马得宏[1] 陈一晖[1] 王宏图[1] 张禄[1] 徐雪美 冉慧粉 

机构地区：[1]昆明医科大学第一附属医院感染科,昆明650032

出　　处：《重庆医学》2014年第17期2152-2154,共3页Chongqing medicine

基　　金：云南省应用基础研究面上项目(2009CD087)

摘　　要：目的探讨美洲大蠊提取物(APA)对实验性肝纤维化组织中核转录因子-κB(NF-κB)和α-平滑肌肌动蛋白(α-SMA)表达的影响,并观察APA的抗纤维化疗效。方法采用四氯化碳复制大鼠肝纤维化模型,同时用0.5g/kg的APA灌胃治疗,测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、清蛋白(Alb)水平。免疫组化测定肝组织中NF-κB和α-SMA表达的水平,并以光镜观察肝病理组织学改变,进行炎性活动度分级及纤维化分期。结果 APA可以降低血清中ALT、AST和肝组织中NF-κB、α-SMA的水平,APA组和三七丹参组与模型组相比血清中Alb水平差异有统计学意义,用药后的肝脏细胞变性坏死及纤维化程度明显降低。结论 APA具有一定的抗纤维化作用,机制可能与其抑制肝组织中NF-κB和α-SMA的表达有关。Objective To investigate the effect of American cockroach extract on the expression of NF-κB andα-SMA in experi-mental liver fibrosis tissue,and to observe its antifibrotic efficacy.Methods The rat model of hepatic fibrosis was duplicated by car-bon tetrachloride and simultaneously given with American cockoach extract 0.5 g/kg by lavage.The level of serum alanine amin-otransferase enzyme （ALT）,aspartate aminotransferase （AST）and albumin （Alb）were determined.The expression levels of NF-κB and a-SMA in liver tissue were determined by the immunohistochemical method and the liver histological changes were observed by light microscopy for conducting the grading of inflammatory activity and the fibrosis classification.Results American cockroach extract could reduce serum ALT,AST and liver tissue NF-κB andα-SMA levels.The serum Alb level had significant difference a-mong the APA group,Pseudo-ginseng Danshen group and the model group.The degeneration and necrosis of liver cells and the fi-brosis degree after medication were significantly reduced.Conclusion The American cockroach extract has certain anti-fibrosis effect,its mechanism may be related with the inhibition of expression of NF-κB andα-SMA.

关 键 词：肝硬化 美洲大蠊提取物 大鼠 核转录因子-ΚB Α-平滑肌肌动蛋白 

分 类 号：R575.2[医药卫生—消化系统]