扇贝多肽对H_2O_2损伤HaCaT细胞的保护作用  

Protective Effect of PCF on H_2O_2-induced HaCaT Cell Injury

作　　者：党转宁 王春波[1] 韩彦弢[1] 

机构地区：[1]青岛大学医学院,山东青岛266071

出　　处：《安徽农业科学》2014年第20期6618-6620,共3页Journal of Anhui Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2011HM046)

摘　　要：[目的]建立H2O2诱导HaCaT细胞氧化应激损伤的体外模型，探讨扇贝多肽（PCF）对H2O2损伤HaCaT细胞的保护作用及其作用机制。[方法]采用不同浓度的H2O2（50、100、200、300、500μmol／L）作用HacaT细胞12h后，采用CCK-8法检测细胞存活率；用不同浓度（1．42、2．84、5．68mmol／L）的PCF分别处理细胞12h后．加入H2O，（300μmol／L）继续作用12h，采用CCK-8法检测细胞活力，采用酶生化法法测定细胞上清液中LDH活性以及细胞质中GSH—Px、T—AOC和CAT水平。l结果l与正常组相比，50～500μmol／L浓度的H2O2依赖性地引起HaCaT细胞氧化损伤，300μmol／L的H2O，使存活率降低到56％（P〈0．01）；与模型组相比，不同浓度的PCF均可保护H2O2诱导的氧化损伤，增加细胞存活率，降低细胞LDH的释放量，提高GSH—Px、T—AOC和CAT含量，增强细胞抗氧化作用。[结论]扇贝多肽能对抗H2O2诱导的氧化应激，对细胞受损具有保护作用，其机制可能与提高抗氧化酶活力有关。[ Objective ] To establish in vitro model of H2O2-induced oxidative stress damage of HaCaT cells, and investigate the polypeptide from Chlamysfarreri＇s protective effect and its possible mechanism on H2O2-indueed damage to HaCaT cells. [ Method] Cell Counting Kit-8 （CCK-8） was used to test the cell survival rate of different concentrations （50, 100,200,300,500 μmol/L） H2O2 for 12 h on HaCaT cells; different concentrations （ 1.42, 2.84, 5.68 mmol/L） PCF treated cell for 12 h, and then the appropriate H202 concentration （300 μmol/L） were adopted to treat, CCK-8 was used to detect the cell viability. Enzyme biochemical method was applied to detect the activity of LDH in culture supernatants, GSH-Px, T-AOC and CAT levels in cell lysis solution. [ Result] Compared with control group, at 50 -500 μmol/L, H2O2 caused concentration-dependent oxidative damage in the HaCaT cell, at 300 μmol./L H2O2 decreased the cell survival rate to 56% （P 〈 0. 01 ） ; Compared with model group, all different concentration PCF protected HaCaT cells from oxidative damage induced by H2O2, significantly increased the cell survival rate, decreased the release of LDH and increase GSH-Px, T-AOC and CAT level, enhanced antioxidation effect of HaCaT cell. [ Conclusion] PCF has the significant protective effects on HaCaT cells injured by H2O2, which may be associated with the increased of antioxidase activity.

关 键 词：扇贝多肽 H2O2 HACAT细胞 氧化应激 

分 类 号：S965.39[农业科学—水产养殖]